Micro pore arrays in free standing cyclic olefin copolymer membranes: fabrication and surface functionalization strategies for in-vitro barrier tissue models

M. Gel; S. Kandasamy; K. Cartledge; C. L. Be; D. Haylock

doi:10.1117/12.2033653

7 December 2013 Micro pore arrays in free standing cyclic olefin copolymer membranes: fabrication and surface functionalization strategies for in-vitro barrier tissue models

M. Gel, S. Kandasamy, K. Cartledge, C. L. Be, D. Haylock

Author Affiliations +

Proceedings Volume 8923, Micro/Nano Materials, Devices, and Systems; 89233E (2013) https://doi.org/10.1117/12.2033653
Event: SPIE Micro+Nano Materials, Devices, and Applications, 2013, Melbourne, Victoria, Australia

Abstract

In recent years there has been growing interest in micro engineered in-vitro models of tissues and organs. These models are designed to mimic the in-vivo like physiological conditions with a goal to study human physiology in an organ-specific context or to develop in-vitro disease models. One of the challenges in the development of these models is the formation of barrier tissues in which the permeability is controlled locally by the tissues cultured at the interface. In-vitro models of barrier tissues are typically created by generating a monolayer of cells grown on thin porous membranes. This paper reports a robust preparation method for free standing porous cyclic olefin copolymer (COC) membranes. We also demonstrate that gelatin coated membranes facilitate formation of highly confluent monolayer of HUVECs. Membranes with thickness in the range of 2-3 um incorporating micro pores with diameter approximately 20 um were fabricated and integrated with microfluidic channels. The performance of the device was demonstrated with a model system mimicking the endothelial barrier in bone marrow sinusoids.

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M. Gel, S. Kandasamy, K. Cartledge, C. L. Be, and D. Haylock "Micro pore arrays in free standing cyclic olefin copolymer membranes: fabrication and surface functionalization strategies for in-vitro barrier tissue models", Proc. SPIE 8923, Micro/Nano Materials, Devices, and Systems, 89233E (7 December 2013); https://doi.org/10.1117/12.2033653

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KEYWORDS

Tissues

In vitro testing

Microfluidics

Silicon

Systems modeling

In vivo imaging

Semiconducting wafers

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