Fluorescence Lifetimes Of Dph Propionyl And Parinaroyl Ether And Ester Analogs Of Choline Glycerophospholipids In Unilamellar Vesicles

Albin Hermetter; Edwin Kalb; Josef Loidl; Fritz Paltauf

doi:10.1117/12.945381

24 June 1988 Fluorescence Lifetimes Of Dph Propionyl And Parinaroyl Ether And Ester Analogs Of Choline Glycerophospholipids In Unilamellar Vesicles

Albin Hermetter, Edwin Kalb, Josef Loidl, Fritz Paltauf

Author Affiliations +

Proceedings Volume 0909, Time-Resolved Laser Spectroscopy in Biochemistry; (1988) https://doi.org/10.1117/12.945381
Event: 1988 Los Angeles Symposium: O-E/LASE '88, 1988, Los Angeles, CA, United States

Abstract

The fluorescence decay of sn-2 diphenylhexatrienylpropionyl- and parinaroyl-labeled choline glycerophospholipids as well as trimethylammonium-DPH (TMA-DPH) were determined by multifrequency phase fluorometry in single bilayer vesicles of diacylglycerophosphocholine (phosphatidylcholine) and its ether analog alkenylacylglycerophosphocholine (choline plasmalogen). The data were analyzed in terms of continuous lifetime distributions using Lorentzian distribution functions. Addition of cholesterol to the bilayers induced narrowing of the lifetime distributions for all labels and unlabeled matrix phospholipids tested. DPH propionyl phospholipids discriminated between ether (plasmalogen) and ester (phosphatidylcholine) phospholipid membranes in that they experienced a much larger lifetime heterogeneity when embedded in the ether lipid, even in the presence or absence of cholesterol.

Citation Download Citation

Albin Hermetter, Edwin Kalb, Josef Loidl, and Fritz Paltauf "Fluorescence Lifetimes Of Dph Propionyl And Parinaroyl Ether And Ester Analogs Of Choline Glycerophospholipids In Unilamellar Vesicles", Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); https://doi.org/10.1117/12.945381

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