In vivo visualization of collagen fiber produced by cultured osteoblasts using sensitive second-harmonic-generation microscopy equipped with a 10-fs mode-locked Ti:sapphire laser

Eiji Hase; Katsuya Sato; Takeshi Yasui

doi:10.1117/12.2079114

5 March 2015 In vivo visualization of collagen fiber produced by cultured osteoblasts using sensitive second-harmonic-generation microscopy equipped with a 10-fs mode-locked Ti:sapphire laser

Eiji Hase, Katsuya Sato, Takeshi Yasui

Author Affiliations +

Proceedings Volume 9329, Multiphoton Microscopy in the Biomedical Sciences XV; 93292Q (2015) https://doi.org/10.1117/12.2079114
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Second-harmonic-generation (SHG) microscopy is a new tool for observing the collagen fiber in tissue in vivo. Conventional SHG microscopy equipped with 100-fs pulse laser is insufficient to visualize low-order-structured, immature collagen with high contrast (for example, collagen fiber produced by cultured cell) because of low nonlinear susceptibility χ⁽²⁾. To enhance the image contrast while avoiding the sample damage, one should increase a peak power of the laser light while maintaining the same average power as the 100-fs laser. In this paper, we constructed sensitive SHG microscopy equipped with a 10-fs Ti:Sapphire laser and succeeded to visualize collagen fibers produced by the cultured osteoblasts in vivo.

Citation Download Citation

Eiji Hase, Katsuya Sato, and Takeshi Yasui "In vivo visualization of collagen fiber produced by cultured osteoblasts using sensitive second-harmonic-generation microscopy equipped with a 10-fs mode-locked Ti:sapphire laser", Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 93292Q (5 March 2015); https://doi.org/10.1117/12.2079114

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