Realtime wavefront sensing in a SPIM microscope, and active aberration tracking

Jonathan M. Taylor; Christopher D. Saunter; Cyril Bourgenot; John M. Girkin; Gordon D. Love

doi:10.1117/12.2080061

31 March 2015 Realtime wavefront sensing in a SPIM microscope, and active aberration tracking

Jonathan M. Taylor, Christopher D. Saunter, Cyril Bourgenot, John M. Girkin, Gordon D. Love

Proceedings Volume 9335, Adaptive Optics and Wavefront Control for Biological Systems; 93350A (2015) https://doi.org/10.1117/12.2080061
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Adaptive optics (AO) can potentially allow high resolution imaging deep inside living tissue, mitigating against the loss of resolution due to aberrations caused by overlying tissue. Closed-loop AO correction is particularly attractive for moving tissue and spatially varying aberrations, but this requires direct wavefront sensing, which in turn requires suitable "guide stars" for use as wavefront references. We present a novel method for generating an orthogonally illuminated guide star suitable for direct wavefront sensing in a wide range of fluorescent biological structures, along with results demonstrating its use for measuring time-varying aberrations, in vivo.

Citation Download Citation

Jonathan M. Taylor, Christopher D. Saunter, Cyril Bourgenot, John M. Girkin, and Gordon D. Love "Realtime wavefront sensing in a SPIM microscope, and active aberration tracking", Proc. SPIE 9335, Adaptive Optics and Wavefront Control for Biological Systems, 93350A (31 March 2015); https://doi.org/10.1117/12.2080061

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