Effects of surface chemistry on the optical properties and cellular interaction of lanthanide-based nanoparticles

Francisco J. Pedraza III; Julio C. Avalos; Lawrence C. Mimun; Brian G. Yust; Andrew Tsin; Dhiraj Kumar Sardar

doi:10.1117/12.2079943

12 March 2015 Effects of surface chemistry on the optical properties and cellular interaction of lanthanide-based nanoparticles

Francisco J. Pedraza III, Julio C. Avalos, Lawrence C. Mimun, Brian G. Yust, Andrew Tsin, Dhiraj Kumar Sardar

Author Affiliations +

Proceedings Volume 9339, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VII; 93390A (2015) https://doi.org/10.1117/12.2079943
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Fluorescent nanoparticles (NPs) such as KYb₂F₇:Tm³⁺ potential in biomedical applications due to their ability to absorb and emit within the biological window, where near infrared light is less attenuated by soft tissue. This results in less tissue damage and deeper tissue penetration making it a viable candidate in biological imaging. Another big factor in determining their ability to perform in a biological setting is the surface chemistry. Biocompatible coatings, including polyethylene glycol (PEG), polyvinylpyrrolidone (PVP), pluronic and folic acid are commonly used because they pose several advantages such as ease of functionalization, better dispersion, and higher cellular uptake. To study the effects of the NP surface chemistry, KYb₂F₇:Tm³⁺ a solvothermal method using PEG, PVP, pluronic acid, and folic acid as a capping agent, followed by thorough optical characterizations. Optical changes were thoroughly studied and compared using absorption, emission, and quantum yield data. Cell viability was obtained by treating Rhesus Monkey Retinal Endothelial cells (RhREC) with KYb₂F₇:Tm³⁺ and counting viable cells following a 24 hour uptake period. The work presented will compare the optical properties and toxicity dependency on the surface chemistry on KYb₂F₇:Tm³⁺. The results will also indicate that KYb₂F₇:Tm₃₊ nanoparticles are viable candidates for various biomedical applications.

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Francisco J. Pedraza III, Julio C. Avalos, Lawrence C. Mimun, Brian G. Yust, Andrew Tsin, and Dhiraj Kumar Sardar "Effects of surface chemistry on the optical properties and cellular interaction of lanthanide-based nanoparticles", Proc. SPIE 9339, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VII, 93390A (12 March 2015); https://doi.org/10.1117/12.2079943

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