Tissue slides analysis using red, green, and blue LEDs as microscope light source

Sebastião Pratavieira; Felipe F. Navascues; Larissa M. de Souza; Ramon G. T. Rosa; Cristina Kurachi; Vanderlei S. Bagnato

doi:10.1117/12.2211702

7 March 2016 Tissue slides analysis using red, green, and blue LEDs as microscope light source

Sebastião Pratavieira, Felipe F. Navascues, Larissa M. de Souza, Ramon G. T. Rosa, Cristina Kurachi, Vanderlei S. Bagnato

Author Affiliations +

Proceedings Volume 9703, Optical Biopsy XIV: Toward Real-Time Spectroscopic Imaging and Diagnosis; 97031X (2016) https://doi.org/10.1117/12.2211702
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

The optical microscopy is one of the most powerful tool in the analysis of biological systems. The usual transmitted light microscope uses a white light lamp as source, what sometimes does not bring optimal results, making it necessary to introduce filters to change some illumination properties like the color temperature or the color itself. There is, of course, an intrinsic limitation on the use of filters that is the lack of an analogical control on the illumination properties and a practical limitation that depends on the number of available filters. To address this need, we developed an illumination system based on (Red, Green and Blue) RGB LEDs, were the microscope operator can control the intensity of each one independently and manually. This paper details the developed system and describes the methods used to compare quantitatively the images acquired while using the standard white light illumination and the images obtained with the developed system. To quantify the contrast, we calculated the relative population standard deviation for the intensities of each channel of the RGB image. This procedure allowed us to compare and understand the major advantages of the developed illumination system. All analysis methods have shown that a contrast enhancement can be obtained under the RGB LEDs light. The presented illumination allowed us to visualize the structures in different samples with a better contrast without the need of any additional optical filters.

Citation Download Citation

Sebastião Pratavieira, Felipe F. Navascues, Larissa M. de Souza, Ramon G. T. Rosa, Cristina Kurachi, and Vanderlei S. Bagnato "Tissue slides analysis using red, green, and blue LEDs as microscope light source", Proc. SPIE 9703, Optical Biopsy XIV: Toward Real-Time Spectroscopic Imaging and Diagnosis, 97031X (7 March 2016); https://doi.org/10.1117/12.2211702

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Light emitting diodes

RGB color model

Microscopes

Optical filters

Lamps

Light sources

Tissue optics

Show All Keywords

Keywords/Phrases

Search In:

Publication Years