Fully integrated reflection-mode photoacoustic/two-photon microscopy in vivo
(Conference Presentation)

Liang Song; Wei Song; Yang Zhang; Wei Zheng

doi:10.1117/12.2214685

27 April 2016 Fully integrated reflection-mode photoacoustic/two-photon microscopy in vivo (Conference Presentation)

Liang Song, Wei Song, Yang Zhang, Wei Zheng

Proceedings Volume 9708, Photons Plus Ultrasound: Imaging and Sensing 2016; 97082H (2016) https://doi.org/10.1117/12.2214685
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Using a water-immersion optical objective in conjunction with a miniature 40-MHz ultrasonic transducer, we developed reflection-mode photoacoustic microscopy with a transverse resolution as high as 320 nm. Here, we further integrated two-photon microscopy capability into the system to enable multimodality in vivo biomedical imaging at submicron resolution. As a result, the system is capable of tri-modality label-free imaging of microvasculature, collagen, and cell morphology, based on the contrast of optical absorption, second-harmonic generation, and autofluorescence, respectively. In addition, we demonstrated simultaneous microscopic imaging of neuron and microvasculature in the brain cortex of a living mouse, which may offer new opportunities for studying the mechanisms of neurovascular coupling.

Conference Presentation

Citation Download Citation

Liang Song, Wei Song, Yang Zhang, and Wei Zheng "Fully integrated reflection-mode photoacoustic/two-photon microscopy in vivo (Conference Presentation)", Proc. SPIE 9708, Photons Plus Ultrasound: Imaging and Sensing 2016, 97082H (27 April 2016); https://doi.org/10.1117/12.2214685

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