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An integrated clinical two photon fluorescence microscope system allows for real-time assessment of freshly excised non-melanoma skin cancer skin biopsies with 2 minutes of preparation and enables imaging of multi-centimeter lesions in under 5 minutes. This system simulates the conventional workflow of a brightfield microscope to minimize pathologist retraining. A blinded study comparing two photon images and coregistered H&E paraffin section images is performed to show degree of concordance between the two modalities.
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Vincent D. Ching-Roa, Chi Z. Huang, Sherrif F. Ibrahim, Michael G. Giacomelli, "Real-time evaluation of freshly excised non-melanoma skin cancer with a clinical two-photon fluorescence microscope," Proc. SPIE PC11944, Multiscale Imaging and Spectroscopy III, PC1194404 (7 March 2022); https://doi.org/10.1117/12.2609236