Phase- and polarization-sensitive full-field optical coherence microscopy for label-free imaging of millisecond scale neural activity

Rishyashring R. Iyer; Yuan-Zhi Liu; Honggu Choi; Carlos A. Renteria; Brian E. Tibble; Stephen A. Boppart

doi:10.1117/12.2609374

7 March 2022 Phase- and polarization-sensitive full-field optical coherence microscopy for label-free imaging of millisecond scale neural activity

Rishyashring R. Iyer, Yuan-Zhi Liu, Honggu Choi, Carlos A. Renteria, Brian E. Tibble, Stephen A. Boppart

Author Affiliations +

Proceedings Volume PC11948, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVI; PC1194807 (2022) https://doi.org/10.1117/12.2609374
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

We present Superfast Polarization-sensitive Off-axis Full-field Optical Coherence Microscopy (SPoOF OCM) as a novel all-optical technique for neurophysiology. Both the optical path length and birefringence induced by the millisecond-scale electrical activity of neurons are captured by SPoOF OCM at 4000 frames per second and with a field-of-view of 200×200 µm sq., 1 µm transverse resolution, 4.5 µm axial resolution, and 300 pm phase sensitivity. With an ability to capture responses spanning three orders of magnitude in both space and time, SPoOF OCM meets the exacting needs of a comprehensive neurophysiology tool and overcomes the existing limitations of traditional electrophysiology and fluorescence microscopy.

Conference Presentation

Citation Download Citation

Rishyashring R. Iyer, Yuan-Zhi Liu, Honggu Choi, Carlos A. Renteria, Brian E. Tibble, and Stephen A. Boppart "Phase- and polarization-sensitive full-field optical coherence microscopy for label-free imaging of millisecond scale neural activity", Proc. SPIE PC11948, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVI, PC1194807 (7 March 2022); https://doi.org/10.1117/12.2609374

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