Three-dimensional visualization of cerebral microhemorrhages and surrounding microvasculature in hypertension

Danny F. Xie; Christian Crouzet; Krystal LoPresti; Yuke Wang; Christopher Robinson; David Cribbs; Mark Fisher; Bernard Choi

doi:10.1117/12.2609926

2 March 2022 Three-dimensional visualization of cerebral microhemorrhages and surrounding microvasculature in hypertension

Danny F. Xie, Christian Crouzet, Krystal LoPresti, Yuke Wang, Christopher Robinson, David Cribbs, Mark Fisher, Bernard Choi

Author Affiliations +

Proceedings Volume PC11966, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIX; PC1196606 (2022) https://doi.org/10.1117/12.2609926
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Cerebral microhemorrhages (CMHs) occur due to ruptures in cerebral microvessels that cause deposits of blood in the brain. Hypertension (HTN) is a major risk factor for CMHs, which have been associated with cognitive decline and ischemic strokes. Despite the clinical significance of CMHs, our understanding of CMH formation remains limited. To address this gap, our group has employed a perfusion-based vascular label with tissue clearing to enable three-dimensional visualization of CMHs with the surrounding microvasculature in HTN mice. Vessel diameters surrounding a CMH were approximately 4.22±0.81 µm. Vessel density in CMH positive tissue regions was approximately 0.083±0.017 µm-1.

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Danny F. Xie, Christian Crouzet, Krystal LoPresti, Yuke Wang, Christopher Robinson, David Cribbs, Mark Fisher, and Bernard Choi "Three-dimensional visualization of cerebral microhemorrhages and surrounding microvasculature in hypertension", Proc. SPIE PC11966, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIX, PC1196606 (2 March 2022); https://doi.org/10.1117/12.2609926

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KEYWORDS

Visualization

Brain

Ischemic stroke

Tissues

Capillaries

Confocal microscopy

MATLAB

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