Label-free multi-photon super-resolution microscopy by multiplexed single point spread function image acquisition

Kayvan Forouhesh Tehrani; Stephen A. Boppart

doi:10.1117/12.2607832

2 March 2022 Label-free multi-photon super-resolution microscopy by multiplexed single point spread function image acquisition

Kayvan Forouhesh Tehrani, Stephen A. Boppart

Author Affiliations +

Proceedings Volume PC11966, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIX; PC119660B (2022) https://doi.org/10.1117/12.2607832
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Image Scanning Microscopy (ISM) super-resolution microscopy has gained momentum for its almost instantaneous improved resolution capabilities. Multiphoton ISM utilizes the shorter emission wavelength and confocal acquisition by exploiting each pixel on the camera as a pinhole, and numerical enhancement to achieve sub-diffraction-limit resolution. We present the use of a multiplexed approach for signal acquisition using a regular EMCCD camera. A spatiotemporal modulation scheme is employed to direct the ultrafast laser pulses to select foci within a field-of-view. Combined with a novel image acquisition method, we show that only 49 images are required to achieve a resolution of 100 nm.

Conference Presentation

Citation Download Citation

Kayvan Forouhesh Tehrani and Stephen A. Boppart "Label-free multi-photon super-resolution microscopy by multiplexed single point spread function image acquisition", Proc. SPIE PC11966, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIX, PC119660B (2 March 2022); https://doi.org/10.1117/12.2607832

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available