Mitochondrial activity studied by Raman spectroscopy

Anna Pieczara; Ewelina Matuszyk; Malgorzata Baranska

doi:10.1117/12.2608819

2 March 2022 Mitochondrial activity studied by Raman spectroscopy

Anna Pieczara, Ewelina Matuszyk, Malgorzata Baranska

Proceedings Volume PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022; PC1197312 (2022) https://doi.org/10.1117/12.2608819
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

The integrity of mitochondrial function is fundamental to cell life, therefore, the detection and understanding of changes taking place in the organelle may allow faster diagnosis. It turns out that Raman spectroscopy is able to detect changes related to mitochondrial dysfunction much faster by tracking changes in redox state of cytochrome. Our research was focused on selecting the appropriate conditions for the use of MitoBADY (2220 cm-1), both in living and fixed endothelial cells. Interestingly, in our research we were able to observe, Raman signature of life band (1604 cm-1) which also characterizes the functioning of mitochondria. The relationship between the 1604 cm-1 and 2220 cm-1 bands also suggests that the reporter can also be an indicator of mitochondrial activity. Acknowledgement: This work was supported by the National Science Centre, Poland, OPUS grant (UMO-2018/29/B/ST4/00335 to MB).

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Citation Download Citation

Anna Pieczara, Ewelina Matuszyk, and Malgorzata Baranska "Mitochondrial activity studied by Raman spectroscopy", Proc. SPIE PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022, PC1197312 (2 March 2022); https://doi.org/10.1117/12.2608819

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KEYWORDS

Raman spectroscopy

Remote sensing

Cell death

Industrial chemicals

Live cell imaging

Oxygen

Physiology

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