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4 March 2022 Quantitative imaging of tyrosine kinase inhibitor distribution in live cells and tissues
Lei G. Wang, Antonio Montaño, Allison Solanki, Nathan McMahon, Kenneth M. Tichauer, Kimberley S. Samkoe, Summer L. Gibbs
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Proceedings Volume PC11979, Frontiers in Biological Detection: From Nanosensors to Systems XIV; PC119790I (2022) https://doi.org/10.1117/12.2612233
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
Intracellular drug target validation and target engagement quantification have proven to be challenging, and all drugs have some degree of non-specific accumulation due to variable drug affinity, biodistribution, pharmacokinetics, and metabolism. Quantification of available drug targets necessitates accounting for both the drug that binds to its target as well as the drug that accumulates in the cells and tissues in a non-specific manner. We have developed a dynamic, fluorescence-based, three-compartment model termed intracellular paired-agent imaging that utilizes fluorophore labeled small molecule therapeutics as imaging agents to measure drug target availability in live cells and tissues.
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Lei G. Wang, Antonio Montaño, Allison Solanki, Nathan McMahon, Kenneth M. Tichauer, Kimberley S. Samkoe, and Summer L. Gibbs "Quantitative imaging of tyrosine kinase inhibitor distribution in live cells and tissues", Proc. SPIE PC11979, Frontiers in Biological Detection: From Nanosensors to Systems XIV, PC119790I (4 March 2022); https://doi.org/10.1117/12.2612233
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KEYWORDS
Tissues
Live cell imaging
Cancer
Luminescence
Molecules
Resistance
Visualization
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