Volumetric imaging of melanoma in situ biopsy using rapid tissue clearing with two-photon fluorescence microscopy

Chi Z. R. Huang; Vincent D. Ching-Roa; Abdalrahman Almallahi; Sherrif F. Ibrahim; Bruce R. Smoller; Michael G. Giacomelli

doi:10.1117/12.2650903

17 March 2023 Volumetric imaging of melanoma in situ biopsy using rapid tissue clearing with two-photon fluorescence microscopy

Chi Z. R. Huang, Vincent D. Ching-Roa, Abdalrahman Almallahi, Sherrif F. Ibrahim, Bruce R. Smoller, Michael G. Giacomelli

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Proceedings Volume PC12352, Photonics in Dermatology and Plastic Surgery 2023; PC1235203 (2023) https://doi.org/10.1117/12.2650903
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We developed a rapid tissue clearing technique combined with two-photon fluorescence microscopy (cFTPM) to image melanoma in situ biopsy volumetrically. cFTPM can provide rapid volumetric melanoma in situ biopsy images with the potential to provide same-day melanoma biopsy turnaround time with full volumetric images. The overall time span of our process, including tissue clearing and imaging, is faster than conventional histology processing.

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Chi Z. R. Huang, Vincent D. Ching-Roa, Abdalrahman Almallahi, Sherrif F. Ibrahim, Bruce R. Smoller, and Michael G. Giacomelli "Volumetric imaging of melanoma in situ biopsy using rapid tissue clearing with two-photon fluorescence microscopy", Proc. SPIE PC12352, Photonics in Dermatology and Plastic Surgery 2023, PC1235203 (17 March 2023); https://doi.org/10.1117/12.2650903

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KEYWORDS

Melanoma

Biopsy

Tissue clearing

Two photon excitation microscopy

Skin cancer

Tissue optics

High dynamic range imaging

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