Uncovering new utility in infrared spectroscopic imaging by pushing the limits of accuracy, speed, and resolution

Rohit Bhargava; Kevin L. Yeh; Seth Kenkel; Yamuna Dilip Phal; Ruo-Jing Ho; Kianoush Falahkheirkhah

doi:10.1117/12.2659474

6 March 2023 Uncovering new utility in infrared spectroscopic imaging by pushing the limits of accuracy, speed, and resolution

Rohit Bhargava, Kevin L. Yeh, Seth Kenkel, Yamuna Dilip Phal, Ruo-Jing Ho, Kianoush Falahkheirkhah

Author Affiliations +

Proceedings Volume PC12373, Optical Biopsy XXI: Toward Real-Time Spectroscopic Imaging and Diagnosis; PC1237303 (2023) https://doi.org/10.1117/12.2659474
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Infrared spectroscopic imaging combines the ability to record molecular content with the ability to visualize chemistry in its spatial diversity. Given the need to record a significantly larger quantity of data than a typical microscopy image (MB vs. GB) and the extensive bandwidth of the spectra (~10 m), trade-offs often have to be made between the closely related considerations of signal to noise ratio, spatial-spectral coverage, resolution and optical arrangements. Here, we present a path from rigorous theory to modeling and design to realizing the advantages offered by new ideas on fundamentally changing these trade-offs. We first describe a new microscope design for increased speed and rapid coverage that is useful for biomedical and clinical tissue imaging. Next, we describe a configuration to measure chirality in samples that promises higher spectral information that present methods. Finally, we present a new approach to nanoscale IR imaging that provides greater fidelity and speed at unprecedented levels of signal to noise ratio. Finally, we show how emerging machine learning approaches can further augment these advances. For each instrumentation advance, examples of use cases will be presented.

Conference Presentation

Citation Download Citation

Rohit Bhargava, Kevin L. Yeh, Seth Kenkel, Yamuna Dilip Phal, Ruo-Jing Ho, and Kianoush Falahkheirkhah "Uncovering new utility in infrared spectroscopic imaging by pushing the limits of accuracy, speed, and resolution", Proc. SPIE PC12373, Optical Biopsy XXI: Toward Real-Time Spectroscopic Imaging and Diagnosis, PC1237303 (6 March 2023); https://doi.org/10.1117/12.2659474

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available