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Diffuse optical spectroscopy is a widely used method for the non-invasive recovery of important biological factors such as tissue oxygenation and total hemoglobin concentration. Frequency domain-diffuse optical spectroscopy improves the accuracy of parameter recovery over continuous wave-diffuse optical spectroscopy by enabling the decoupling of tissue absorption from tissue scattering. However, this comes at the price of increased instrumentation cost and complexity. Here we detail an easy to build, low-cost, and robust frequency domain-diffuse optical spectroscopy system to increase accessibility to this technology along with testing of the system’s stability and accuracy to ensure its applicability for biological measurements.
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Joseph B. Majeski, Vincent D. Ching-Roa, Michael G. Giacomelli, Regine Choe, "Novel design of a low-cost heterodyne frequency domain-diffuse optical spectroscopy instrument," Proc. SPIE PC12376, Optical Tomography and Spectroscopy of Tissue XV, PC123760C (7 March 2023); https://doi.org/10.1117/12.2649249