Quantitative analysis of collagen organization from second-harmonic generation images (Conference Presentation)

Pedro Guimarães; Maria Mateus; António Miguel Morgado; Ana Batista

doi:10.1117/12.2645686

15 March 2023 Quantitative analysis of collagen organization from second-harmonic generation images (Conference Presentation)

Pedro Guimarães, Maria Mateus, António Miguel Morgado, Ana Batista

Author Affiliations +

Proceedings Volume PC12384, Multiphoton Microscopy in the Biomedical Sciences XXIII; PC1238407 (2023) https://doi.org/10.1117/12.2645686
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Second-harmonic generation (SHG) is uniquely capable of imaging collagen non-invasively with high-resolution, making it ideal to evaluate tissue organization in health and disease. For this, quantitative data analysis is essential. Different approaches have been proposed to quantify tissue organization from SHG images. Nevertheless, these methods have never been objectively evaluated or compared. In this study, we performed a comprehensive analysis on the performance of different metrics in computer-generated SHG images with increasing levels of disorganization to evaluate the advantages and limitations of each approach.

Conference Presentation

Citation Download Citation

Pedro Guimarães, Maria Mateus, António Miguel Morgado, and Ana Batista "Quantitative analysis of collagen organization from second-harmonic generation images (Conference Presentation)", Proc. SPIE PC12384, Multiphoton Microscopy in the Biomedical Sciences XXIII, PC1238407 (15 March 2023); https://doi.org/10.1117/12.2645686

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