Frequency-multiplexed aberration measurement and correction for confocal microscopy (Conference Presentation)

Daisong Pan; Xinxin Ge; Lydia Liu; Leah Ferger; Ehud Isacoff; Na Ji

doi:10.1117/12.2667847

16 March 2023 Frequency-multiplexed aberration measurement and correction for confocal microscopy (Conference Presentation)

Daisong Pan, Xinxin Ge, Lydia Liu, Leah Ferger, Ehud Isacoff, Na Ji

Author Affiliations +

Proceedings Volume PC12388, Adaptive Optics and Wavefront Control for Biological Systems IX; PC1238806 (2023) https://doi.org/10.1117/12.2667847
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

In single-photon confocal fluorescence microscopy, sample-induced optical aberrations affect both excitation and detection paths and can lead to severely degraded image quality. We applied an adaptive optics (AO) method based on frequency-multiplexed aberration measurement to a confocal microscope. We validated this method by performing AO correction on features of different sizes with artificial aberrations and demonstrating drastic improvement in image signal, contrast, and resolution. Moreover, we showed that this method can significantly enhance imaging performance through living zebrafish larvae and in the mouse brain in vivo.

Conference Presentation

Citation Download Citation

Daisong Pan, Xinxin Ge, Lydia Liu, Leah Ferger, Ehud Isacoff, and Na Ji "Frequency-multiplexed aberration measurement and correction for confocal microscopy (Conference Presentation)", Proc. SPIE PC12388, Adaptive Optics and Wavefront Control for Biological Systems IX, PC1238806 (16 March 2023); https://doi.org/10.1117/12.2667847

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