Deep refractive index tomography by partial reconstruction and wave-backpropagation (Conference Presentation)

Osamu Yasuhiko; Kozo Takeuchi

doi:10.1117/12.2646113

16 March 2023 Deep refractive index tomography by partial reconstruction and wave-backpropagation (Conference Presentation)

Osamu Yasuhiko, Kozo Takeuchi

Author Affiliations +

Proceedings Volume PC12389, Quantitative Phase Imaging IX; PC123890I (2023) https://doi.org/10.1117/12.2646113
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Visualization of refractive index (RI) distribution of thick specimens is challenging due to multiple-scattering and sample-induced aberration caused by the inhomogeneity of the sample. Here, we propose a novel RI reconstruction method that overcoming these detrimental effects by partial reconstruction of RI distribution and computational wave backpropagation through the partial RI distribution. Exploiting this computational approach, we demonstrate the subcellular-resolution visualization of multicellular spheroid with a diameter of 140 µm. We further introduce the latest results on various cell-type spheroids with different morphology that demonstrate the generalizability of this approach, and discuss applicability to biomedical fields.

Conference Presentation

Citation Download Citation

Osamu Yasuhiko and Kozo Takeuchi "Deep refractive index tomography by partial reconstruction and wave-backpropagation (Conference Presentation)", Proc. SPIE PC12389, Quantitative Phase Imaging IX, PC123890I (16 March 2023); https://doi.org/10.1117/12.2646113

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