Near infrared fluorophore specific for annexin A2 identifies peripheral nerve injury in a rodent crush model (Conference Presentation)

David Brogan; Tony Lee; Jason Wever; Christopher Dy; Samuel Achilefu

doi:10.1117/12.2655718

17 March 2023 Near infrared fluorophore specific for annexin A2 identifies peripheral nerve injury in a rodent crush model (Conference Presentation)

David Brogan, Tony Lee, Jason Wever, Christopher Dy, Samuel Achilefu

Author Affiliations +

Proceedings Volume PC12398, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XIV; PC123980J (2023) https://doi.org/10.1117/12.2655718
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Increased permeability in the blood nerve barrier is found after peripheral nerve injury(1) and annexin A-2 has been shown to translocate to Schwann cell surface after injury(2). We hypothesize that this change in permeability can facilitate intraneaural transit of LS-301 to identify nerve injury. We subjected Lewis rats to a mild or severe unilateral sciatic nerve crush injury and performed NIR imaging after administration of Cypate-3 dye and LS-301. Fluorescence within the ROI of the mildly injured nerve and severely injured nerve was significantly increased compared to their contralateral control at 5 hrs after injury. It appears that intravenous administration of near-infrared fluorophores results in detectable changes in nerve fluorescence compared to baseline.

Conference Presentation

Citation Download Citation

David Brogan, Tony Lee, Jason Wever, Christopher Dy, and Samuel Achilefu "Near infrared fluorophore specific for annexin A2 identifies peripheral nerve injury in a rodent crush model (Conference Presentation)", Proc. SPIE PC12398, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XIV, PC123980J (17 March 2023); https://doi.org/10.1117/12.2655718

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