PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
Macroscopic fluorescence lifetime Forster resonance energy transfer (MFLI FRET) imaging presents an analytical tool to non-invasively quantify drug-receptor engagement in tumors and other organs in preclinical studies. Near infrared (NIR) fluorescence lifetime FRET acts as a direct reporter of drug-target engagement via measuring the fraction of labeled-donor antibody drugs undergoing binding to their respective target receptor using in vitro microscopy or in vivo macroscopy. We show that NIR MFLI FRET measurements correlates with drug-receptor binding in tumor cells, but strikingly, not with ubiquitously used ex vivo receptor expression assessment. Our study demonstrates that MFLI FRET is a powerful non-invasive imaging approach to study the impact of tumor microenvironment on drug delivery and drug-target engagement in intact live tumor xenografts.
Amit Verma,Catherine Sherry,Vikas Pandey,Nanxue Yuan,John Williams,Xavier Intes, andMargarida M. Barroso
"Macroscopy fluorescence lifetime FRET monitors drug-target engagement in tumors in vivo", Proc. SPIE PC12821, Visualizing and Quantifying Drug Distribution in Tissue VIII, PC128210F (13 March 2024); https://doi.org/10.1117/12.3008749
ACCESS THE FULL ARTICLE
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
The alert did not successfully save. Please try again later.
Amit Verma, Catherine Sherry, Vikas Pandey, Nanxue Yuan, John Williams, Xavier Intes, Margarida M. Barroso, "Macroscopy fluorescence lifetime FRET monitors drug-target engagement in tumors in vivo," Proc. SPIE PC12821, Visualizing and Quantifying Drug Distribution in Tissue VIII, PC128210F (13 March 2024); https://doi.org/10.1117/12.3008749