On-chip Raman spectroscopy of live single cells for the staging of oesophageal adenocarcinoma progression

Alisha Farooq; Christopher D. Wood; John E. Ladbury; Stephen D. Evans

doi:10.1117/12.3008976

13 March 2024 On-chip Raman spectroscopy of live single cells for the staging of oesophageal adenocarcinoma progression

Alisha Farooq, Christopher D. Wood, John E. Ladbury, Stephen D. Evans

Proceedings Volume PC12839, Biomedical Vibrational Spectroscopy 2024: Advances in Research and Industry; PC128390G (2024) https://doi.org/10.1117/12.3008976
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

The absence of early diagnosis contributes to oesophageal cancer being the sixth most common cause of global cancer-associated deaths, with a five-year survival rate of <20%. Barrett’s oesophagus (BO) is the main pre-cancerous condition to oesophageal adenocarcinoma (OAC) development, characterised by the morphological transition of oesophageal squamous epithelium to metaplastic columnar epithelium. Current diagnostic methods involve invasive techniques such as endoscopies, and with few identified biomarkers of disease progression, OAC detection is costly and challenging. In this work, single-cell Raman spectroscopy was combined with microfluidic techniques to characterise the development of OAC through the progression of healthy epithelial, BO and OAC cell lines. Principal component analysis and linear discriminant analysis were used to classify the different stages of cancer progression. with the ability to differentiate between healthy and cancerous cells with an accuracy of 97%. These results highlight the potential for rapid and reliable diagnostic screening of BO patients.

Conference Presentation

Citation Download Citation

Alisha Farooq, Christopher D. Wood, John E. Ladbury, and Stephen D. Evans "On-chip Raman spectroscopy of live single cells for the staging of oesophageal adenocarcinoma progression", Proc. SPIE PC12839, Biomedical Vibrational Spectroscopy 2024: Advances in Research and Industry, PC128390G (13 March 2024); https://doi.org/10.1117/12.3008976

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KEYWORDS

Raman spectroscopy

Cancer

Cancer detection

Diseases and disorders

Diagnostics

Endoscopy

Microfluidics

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