Quantitative phase imaging of nanoscale ultrastructure for analyzing mechanical properties of live cells

Adam P. Wax

doi:10.1117/12.3003417

13 March 2024 Quantitative phase imaging of nanoscale ultrastructure for analyzing mechanical properties of live cells

Adam P. Wax

Proceedings Volume PC12844, Optical Elastography and Tissue Biomechanics XI; PC128440O (2024) https://doi.org/10.1117/12.3003417
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

The nanoscale ultrastructure of cells can be analyzed using quantitative phase imaging to examine variations in refractive index. By studying cell response to mechanical stimuli, these refractive index variations can be related to viscoelastic properties such as shear modulus as determined by atomic force microscopy. In contrast, the spatial organization of these variations is characterized using fractal dimension which is seen to increase with cancer progression. Our recent work has linked these two measurements using multiscale measurements of optical phase variance to calculate disorder strength and in turn to determine the fractal dimension of the structures. Disorder strength is seen to vary with imaging resolution with fractal dimension revealed in the observed trends over length scale. Studies will be presented which enable distinction of cell phenotype using these two parameters while their combined use presents a new approach for better understanding cellular restructuring. Finally, modulation of these parameters is examined as a means to study early carcinogenic due to heavy metal exposure.

Conference Presentation

Citation Download Citation

Adam P. Wax "Quantitative phase imaging of nanoscale ultrastructure for analyzing mechanical properties of live cells", Proc. SPIE PC12844, Optical Elastography and Tissue Biomechanics XI, PC128440O (13 March 2024); https://doi.org/10.1117/12.3003417

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