Deep insights from 3D FLIM: metabolic FLIM analysis taken to the 3rd dimension

Lukas Braun; Julius Heitz; Taravat Saeb-Gilani; Hauke Studier; Wolfgang Becker

doi:10.1117/12.3005165

13 March 2024 Deep insights from 3D FLIM: metabolic FLIM analysis taken to the 3rd dimension

Lukas Braun, Julius Heitz, Taravat Saeb-Gilani, Hauke Studier, Wolfgang Becker

Proceedings Volume PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV; PC128470X (2024) https://doi.org/10.1117/12.3005165
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Fluorescence lifetime imaging (FLIM) is a versatile tool, even detecting cancers without staining agent from NADH and FAD autofluorescence. While confocal techniques provide wonderful images of 2D structures, they risk neglecting crucial details in the out-of-image-plane dimension. Thorough understanding of three-dimensional samples is obtained through “Z-stack measurements”, coupled with appropriate functional sectioning capabilities. Here, we demonstrate the combination of Becker&Hickl’s advanced SPCimage NG data analysis suite with an easy-to-follow ImageJ/Fiji 3D rendering workflow to generate volume FLIM images of real samples. Combined with Becker&Hickl’s renowned lifetime measurement precision 3D FLIM elevates metabolic imaging and deep-tissue work.

Conference Presentation

Citation Download Citation

Lukas Braun, Julius Heitz, Taravat Saeb-Gilani, Hauke Studier, and Wolfgang Becker "Deep insights from 3D FLIM: metabolic FLIM analysis taken to the 3rd dimension", Proc. SPIE PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV, PC128470X (13 March 2024); https://doi.org/10.1117/12.3005165

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Fluorescence lifetime imaging

3D image processing

3D metrology

Data analysis

Autofluorescence

Cancer

Cancer detection

Show All Keywords

Keywords/Phrases

Search In:

Publication Years