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Most two photon and confocal microscopes are limited by the sequential detection of pixels. I present recent work using low-cost silicon photomultiplier (SiPM) technology to build arrays of single-photon sensitive detectors. I present both spatially multiplexed imaging in highly scattering tissue and hyperspectral detection using 16 parallel SiPMs in two detector configurations. These enable 200 MP/s imaging through 4 spectral channels or hyperspectral read out at 50 MP/s and 20 nm spectral resolution at very low cost.
Vincent D Ching-Roa andMichael G. Giacomelli
"Accelerating two-photon fluorescence imaging rates and spectral throughput using arrays of single photon sensitive detectors", Proc. SPIE PC12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI, PC1284809 (13 March 2024); https://doi.org/10.1117/12.3003972
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Vincent D Ching-Roa, Michael G. Giacomelli, "Accelerating two-photon fluorescence imaging rates and spectral throughput using arrays of single photon sensitive detectors," Proc. SPIE PC12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI, PC1284809 (13 March 2024); https://doi.org/10.1117/12.3003972