Towards in-vivo, non-invasive, label-free, real-time 3D H&E imaging for clinical applications via 3D phase contrast from quantitative oblique back illumination microcopy (qOBM)

Francisco E. Robles; Richard Levenson; Tanishq M. Abraham; Paloma Casteleiro Costa; Zhe Guang; Caroline E. Serafini

doi:10.1117/12.3008577

13 March 2024 Towards in-vivo, non-invasive, label-free, real-time 3D H&E imaging for clinical applications via qOBM

Francisco E. Robles, Richard Levenson, Tanishq M. Abraham, Paloma Casteleiro Costa, Zhe Guang, Caroline E. Serafini

Proceedings Volume PC12852, Quantitative Phase Imaging X; PC128520Z (2024) https://doi.org/10.1117/12.3008577
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Quantitative oblique-back-illumination microscopy (qOBM) enables quantitative phase imaging (QPI) with epi-illumination, and thus permits the use of phase contrast in applications that were previously out-of-reach for QPI, including clinical medicine. Here, I will discuss our latest efforts to apply qOBM for clinical applications, specifically tissue imaging for non-invasive diagnostics and image guided therapy. Our approach uses an unsupervised cycle generative adversarial networks to translate 3D phase images of thick fresh tissues to appear like H&E-stained tissue sections. This work paves the way for non-invasive, label-free, real-time 3D H&E imaging which can be transformative for disease detection and guided therapy.

Conference Presentation

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Francisco E. Robles, Richard Levenson, Tanishq M. Abraham, Paloma Casteleiro Costa, Zhe Guang, and Caroline E. Serafini "Towards in-vivo, non-invasive, label-free, real-time 3D H&E imaging for clinical applications via qOBM", Proc. SPIE PC12852, Quantitative Phase Imaging X, PC128520Z (13 March 2024); https://doi.org/10.1117/12.3008577

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