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Compressed sensing has emerged as a promising technique for high-speed 3-D imaging. Recent work proposed light field tomography (LIFT) to acquire en-face one-dimension (1-D) projections instead of 2-D images and reformulate as a computed tomography problem. The light field with reduced dimension brings high temporal resolution and synthetic refocusing ability in post-processing. We hereby propose a scanning light sheet system with LIFT detection, specifically tailored for achieving kilohertz 3-D fluorescent microscopy. The selective illumination introduces signal sparsity, and thus better reconstruction quality for the compressive detection system. And the high-speed light field imager replaces the active focusing unit in scanning light sheet system and increases the volume rate of 3D detection.
Zhaoqiang Wang,Tzung K. Hsiai, andLiang Gao
"Kilohertz 3-D fluorescent microscopy with light field tomography", Proc. SPIE PC12857, Computational Optical Imaging and Artificial Intelligence in Biomedical Sciences, PC128571E (13 March 2024); https://doi.org/10.1117/12.3001953
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Zhaoqiang Wang, Tzung K. Hsiai, Liang Gao, "Kilohertz 3-D fluorescent microscopy with light field tomography," Proc. SPIE PC12857, Computational Optical Imaging and Artificial Intelligence in Biomedical Sciences, PC128571E (13 March 2024); https://doi.org/10.1117/12.3001953