Label-free interferometric scattering microscopy and spectroscopy with single-molecule sensitivity

Marek Piliarik

doi:10.1117/12.3022610

20 June 2024 Label-free interferometric scattering microscopy and spectroscopy with single-molecule sensitivity

Marek Piliarik

Proceedings Volume PC13008, Biophotonics in Point-of-Care III; PC1300806 (2024) https://doi.org/10.1117/12.3022610
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Abstract

Interferometric scattering microscopy is a breakthrough in ultrasensitive, label-free detection of biomolecules enabled by fast and sensitive imaging of light scattered by weakly scattering objects. Our research focuses on discerning subtle fluctuations in the scattering signal to describe biomolecular interactions and processes hidden deep within the subdiffractional volume of the probe beam. We demonstrate how the fluctuation in the scattering amplitude can be associated with conformation changes taking place at the level of a single, or a few unlabeled biomolecules and open new possibilities for the next generation of super-resolution microscopy techniques. We further combine the ultrasensitive detection of single molecules with real-time Raman spectroscopy to monitor the structural fluctuations on the single-molecule level. Understanding the dynamics of biological matter opens new avenues in label-free super-resolution microscopy and ultimately sensitive detection and identification of biomolecular samples.

Conference Presentation

Citation Download Citation

Marek Piliarik "Label-free interferometric scattering microscopy and spectroscopy with single-molecule sensitivity", Proc. SPIE PC13008, Biophotonics in Point-of-Care III, PC1300806 (20 June 2024); https://doi.org/10.1117/12.3022610

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Raman scattering

Interferometry

Light scattering

Microscopy

Biomolecules

Proteins

Single molecule spectroscopy

Show All Keywords

Keywords/Phrases

Search In:

Publication Years