Use of picosecond Kerr-gated Raman spectroscopy to suppress signals from both surface and deep layers in bladder and prostate tissue

Maria Consuelo Hart Prieto; Pavel Matousek; Michael Towrie; Anthony William Parker; Mark P.J. Wright; Alastair Ritchie M.D.; Nicholas Stone

doi:10.1117/1.1991848

1 July 2005 Use of picosecond Kerr-gated Raman spectroscopy to suppress signals from both surface and deep layers in bladder and prostate tissue

Maria Consuelo Hart Prieto, Pavel Matousek, Michael Towrie, Anthony William Parker, Mark P.J. Wright, Alastair Ritchie M.D., Nicholas Stone

Author Affiliations +

Journal of Biomedical Optics, Vol. 10, Issue 4, 044006 (July 2005). https://doi.org/10.1117/1.1991848

Abstract

Raman spectroscopy is an optical technique able to interrogate biological tissues, giving us an understanding of the changes in molecular structure that are associated with disease development. The Kerr-gated Raman spectroscopy technique uses a picosecond pulsed laser as well as fast temporal gating of collected Raman scattered light. Prostate samples for this study were obtained by taking a chip at the transurethral resection of the prostate (TURP), and bladder samples from a biopsy taken at transurethral resection of bladder tumor (TURBT) and TURP. Spectra obtained through the bladder and prostate gland tissue, at different time delays after the laser pulse, clearly show change in the spectra as depth profiling occurs, eventually showing signals from the uric acid cell and urea cell, respectively. We show for the first time, using this novel technique, that we are able to obtain spectra from different depths through both the prostate gland and the bladder. This has major implications in the future of Raman spectroscopy as a tool for diagnosis. With the help of Raman spectroscopy and Kerr gating, it may be possible to pick up the spectral differences from a small focus of adenocarcinoma of the prostate gland in an otherwise benign gland, and also stage the bladder cancers by assessing the base of the tumor post resection.

©(2005) Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation Download Citation

Maria Consuelo Hart Prieto, Pavel Matousek, Michael Towrie, Anthony William Parker, Mark P.J. Wright, Alastair Ritchie M.D., and Nicholas Stone "Use of picosecond Kerr-gated Raman spectroscopy to suppress signals from both surface and deep layers in bladder and prostate tissue," Journal of Biomedical Optics 10(4), 044006 (1 July 2005). https://doi.org/10.1117/1.1991848

Published: 1 July 2005

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY