Optical characterization of Pseudomonas fluorescens on meat surfaces using time-resolved fluorescence

Alain Bouchard; Julie Frechette; Marcia L. Vernon; Jean-François Cormier; Rene M. Beaulieu; Réal Vallée; Akier Assanta Mafu

doi:10.1117/1.2162166

1 January 2006 Optical characterization of Pseudomonas fluorescens on meat surfaces using time-resolved fluorescence

Alain Bouchard, Julie Frechette, Marcia L. Vernon, Jean-François Cormier, Rene M. Beaulieu, Réal Vallée, Akier Assanta Mafu

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Journal of Biomedical Optics, Vol. 11, Issue 1, 014011 (January 2006). https://doi.org/10.1117/1.2162166

Abstract

A scanning optical system for the detection of bacteria on meat surfaces based on fluorescence lifetime and intensity measurements is described. The system detects autofluorescent light emitted by naturally occurring fluorophores in bacteria. The technique only requires minimal sample preparation and handling, thus the chemical properties of the specimen are preserved. This work presents the preliminary results obtained from a time-resolved fluorescence imaging system for the characterization of a nonpathogenic gram-negative bacteria, Pseudomonas fluorescens. Initial results indicate that the combination of fluorescence lifetime and intensity measurements provides a means for characterizing biological media and for detecting microorganisms on surfaces.

©(2006) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Alain Bouchard, Julie Frechette, Marcia L. Vernon, Jean-François Cormier, Rene M. Beaulieu, Réal Vallée, and Akier Assanta Mafu "Optical characterization of Pseudomonas fluorescens on meat surfaces using time-resolved fluorescence," Journal of Biomedical Optics 11(1), 014011 (1 January 2006). https://doi.org/10.1117/1.2162166

Published: 1 January 2006

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Cited by 5 scholarly publications and 15 patents.

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KEYWORDS

Luminescence

Data modeling

Bacteria

Biomedical optics

Imaging systems

Time resolved spectroscopy

Microorganisms

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