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1.IntroductionThe rupture of atherosclerotic plaque is a frequent precursor to thrombus-mediated acute coronary syndromes and is responsible for almost 75% of acute myocardial infarctions in patients. 1, 2, 3, 4, 5 One type of atherosclerotic plaque, the necrotic-core fibroatheroma (NCFA), is most commonly found at the site of coronary thrombosis in patients who have succumbed to sudden cardiac death.6 Autopsy studies have demonstrated that NCFAs, which are comprised of a thin, mechanically weak fibrous cap overlying a compliant necrotic lipid-rich core, have a high propensity for rupture (Fig. 1 ).6 The fibrous cap, predominantly composed of collagen and smooth muscle cells, is an important structural entity that determines the mechanical stability of the NCFA. It provides a protective envelope that shields the highly thrombogenic material in the necrotic core from contact with coagulation factors in the coronary blood circulation.7 The onset of occlusive coronary thrombosis is initiated by the fracture of the fibrous cap, followed by the release of thrombogenic contents of the plaque into the circulation. Recent studies have shown that lipid-lowering therapy initiates dynamic remodeling of the plaque by causing a net increase in the collagen content and thickness of the fibrous cap, ultimately resulting in the stabilization of the plaque.8, 9, 10 Due to its role in plaque stability, the fibrous cap is an important anatomic target for the detection of high-risk atherosclerotic plaques in patients. Fig. 1Histological section showing a necrotic core fibroatheroma (NCFA) with a thin fibrous cap (arrow) overlying a large necrotic lipid core (NC). The fibrous cap, predominantly composed of collagen and smooth muscle cells, is an important structural entity that determines the mechanical stability of the NCFA. Masson's trichrome; original magnification . Scale bar, . ![]() Laser speckle imaging (LSI) is a new optical technique that has demonstrated high sensitivity and specificity for atherosclerotic plaque diagnosis.11, 12 In this technique, a focused coherent laser beam illuminates the plaque, time-varying laser speckle images are acquired, and the speckle pattern decorrelation times are computed. Since laser speckle patterns are modulated by the random Brownian motion of particles within the plaque, the measurement of speckle decorrelation times provides information on plaque viscoelasticity. In a previous study to demonstrate LSI for plaque characterization, speckle decorrelation time constants were found to be highly dependent on plaque type and composition, including lipid and collagen content.11 In this prior work, the decorrelation time constants were computed over the entire speckle pattern. As a result, the effects of Brownian motion were integrated over the illuminated volume, and depth-resolved spatial information was lost. Due to the diffusion properties of light propagation in tissue, photons returning from deeper regions within the tissue have a higher probability of remittance farther away from the illumination beam entry point. 13, 14, 15, 16 By investigating speckle pattern decorrelation as a function of distance from the illumination beam entry point, it therefore should be possible to obtain information on plaque morphology as a function of depth. In this work, we consider this feature of LSI and explore its ability to estimate fibrous cap thickness in NCFAs. Using ex vivo studies, we investigate the possibility of combining spatio-temporal measurements of laser speckle fluctuations with diffusion theory of light propagation in tissues to obtain information about fibrous cap thickness. 2.Methods2.1.Laser Speckle ImagingLaser speckle imaging was conducted on thoracic and abdominal aortic segments, obtained from 14 human cadavers. Immediately after harvest, the aortas were stored in phosphate buffered saline (PBS) at . A total of 118 aortic plaques were randomly selected by gross examination, and imaging was performed within a PBS bath maintained at . The time between autopsy and imaging did not exceed . A bench-top LSI system was constructed to acquire laser speckle images of aortic plaques ex vivo. Light from a polarized helium neon laser was reflected off a galvanometer mounted mirror, expanded by 5:1, and then focused to a spot on the luminal surface of the plaque. The distance between the focusing lens and the aortic specimen was . The computer-controlled galvanometer-mounted mirror enabled accurate positioning of the illumination beam over the center of the plaque. Cross-polarized laser speckle images were acquired using a charge-coupled device (CCD) camera (TM-6710CL, Pulnix, Sunnyvale, California). Time-varying laser speckle images were obtained from each imaging site at for a duration of . To ensure accurate registration with histology, the right and left lateral edges of the remitted speckle pattern diameter were marked with India ink. Following imaging, plaques were fixed in 10% formalin, embedded, and sectioned for histological processing. Sections were cut across the fiducial ink marks and stained with hematoxylin-eosin and Trichrome stains. The histological sections were interpreted by a pathologist blinded to the LSI data. Plaques that were histologically confirmed as NCFAs were selected for subsequent analysis. Morphometric measurements of fibrous cap thickness were obtained from the digitized histopathology slides. In each digitized histological image, the position of the illumination spot was delineated as the midpoint between the fiducial marks. Fibrous cap thickness was measured by starting at the midpoint and moving outward toward the fiducial marks on either side. The average fibrous cap thicknesses for the right and left portions of the NCFA were computed from cap thickness measurements performed at spacings on either side of the illumination position. Hence for each NCFA, two histological measurements of average cap thickness were obtained, each corresponding to portions of the plaque to the right and left of the illumination site. 2.2.Laser Speckle Imaging - Monte Carlo AnalysisWe have previously shown that the rate of speckle pattern decorrelation, governed by the Brownian motion of intrinsic particles, is dependent on the viscoelastic properties of the plaque, and hence the plaque composition. A NCFA, in simplest terms, can be described as a two-layered tissue with a stiffer fibrous layer, rich in collagen and smooth muscle cells, overlying a deeper core of lower viscosity comprising lipid and necrotic debris [Fig. 2a ]. Based on Brownian motion considerations, we expect that the fibrous layer would affect a slower rate of speckle decorrelation (longer time constant) compared to the necrotic lipid layer (shorter time constant). Monte Carlo simulations and diffusion theory studies have shown that as photons travel deeper into tissue, they have a higher probability of being remitted farther away from the illumination location14, 17, 18 [Fig. 2a]. Due to this effect, in a NCFA, when speckle decorrelation is measured as a function of radial distance, , from the illumination location, a radially dependent time constant, , will be observed. In proximity to the illumination location, most photons will only have traversed the stiff, high-viscosity fibrous cap and the decorrelation times will be long. Farther away from the illumination site, the majority of photons will have propagated through the deeper low viscosity core, and the decorrelation times will therefore be shorter [Fig. 2b]. We hypothesize that the distance, , at which this time constant transition occurs, will be correlated to the NCFA cap thickness (Fig. 2). Fig. 2(a) Radially resolved speckle decorrelation time constant is plotted versus distance from the source. The inflection distance is shown in the plot. At distances less than , most photons will only have traversed the fibrous cap and the decorrelation times will be higher. In contrast, at distances greater than , the majority of photons will have propagated through the necrotic core and the decorrelation times will be shorter. (b) Schematic of photon propagation through two-layer model demonstrating the approximate locations of and . ![]() To test this hypothesis, we analyzed the spatio-temporal characteristics of LSI data obtained from NCFAs. For each speckle image series, the position of the illumination spot, which was approximately at the center of the plaque, was manually located. Speckle decorrelation curves as a function of time were obtained for each value of by performing a normalized windowed cross-correlation centered at each . Each window in the time series was correlated with the first image window in the Fourier domain.19 For each value of , a normalized speckle decorrelation curve was created by extracting windowed cross-correlation maxima and normalizing them to the windowed autocorrelation maxima. The radially resolved decorrelation time constant was computed by exponential fitting of the decorrelation curve for each , and by moving the center of the window in increments of . The window was translated from the illumination spot to the ink mark locations for accurate registration with histology. Due to tissue heterogeneity and variations in fibrous cap thickness, the measured laser speckle patterns were asymmetric; hence, we obtained two plots of versus corresponding to either side of the illumination location for each NCFA, and the inflection distance, , is measured from each plot. Therefore, a total of 38 measurements from 19 NCFAs were obtained. We approximated that at distances smaller than , the observed speckle pattern was predominantly affected by photon scattering within the fibrous cap (Fig. 2). Conversely, for distances greater than , the observed speckle pattern was primarily affected by scattering in the necrotic core (Fig. 2). To determine the value of that best approximates the inflection distance, was measured as the distance at which dropped to the range of 80 to 40% of its maximum value at the illumination location. Therefore, multiple values of were determined over the range of thresholds of 80 to 40% of . Next, we related the radial distance, , measured for the range of thresholds of to the thickness of the fibrous cap, by combining a diffusion theory model of spatially resolved diffuse reflectance20 and a Monte-Carlo model of light transport in tissue14 to estimate the radially resolved maximum photon penetration depth through the NCFA fibrous cap. The tissue was described by its optical parameters: the absorption coefficient , the scattering coefficient , and the anisotropy coefficient , as well as the refractive indices of air and tissue . First, we derived the optical properties of fibrous tissue at by measuring the radially dependent remittance from six atherosclerotic plaques, histologically confirmed as fibrous plaques. Fibrous plaques were utilized, as the optical properties of collagen in these plaques should closely resemble the optical properties of fibrous caps in NCFAs. Time-varying speckle images of the fibrous plaques were obtained using the imaging set up as described before. Given the quantum efficiency and gain of the CCD camera, the total number of diffuse photons remitted from the plaque and detected by the CCD sensor was measured by time-averaging speckle images acquired over a period of . The radially resolved photon probability for each fibrous plaque was generated by summing the number of photons detected over different annuli of radii , and then normalizing this value by the total number of photons detected over the area of the CCD detector. The theoretical radial photon probabilities calculated from a single-scatterer diffusion model for the case of a semi-infinite homogeneous tissue20 were fitted to the measured radial photon probabilities, , by means of a least-square optimization procedure to extract the optical properties, , , and , for each fibrous plaque. Next, we compared optical properties obtained from the fibrous plaques to that derived from time-averaged laser speckle images of five normal aortic tissue samples at wavelength. The optical properties of the fibrous plaques were used as inputs to a Monte Carlo model that assumed a semi-infinite homogeneous layer.14 Photon initial conditions included input beams perpendicular to the semi-infinite layer. Multiple runs were performed with the same set of optical properties, and a total of 500,000 photon packet trajectories were launched. Remitted photons were collected over a radial distance of . From the output of the Monte Carlo simulations, the radially resolved maximum penetration depth was recorded for each photon. The mean of the distribution of maximum penetration depths of photons remitted at a distance provided an estimate of the mean maximum penetration depth, , as a function of radial distance.14 Similarly, look-up tables were obtained using Monte Carlo (MC) simulations for each of the six fibrous plaques. Finally, for each NCFA, was determined via spatio-temporal LSI as described before, and input into each of the six look-up tables to obtain a parameter . The mean value was calculated for each NCFA as the average of six values. Consequently, was calculated for corresponding to the range of inflection thresholds of . The parameter obtained using the combined LSI-MC technique for each NCFA was compared with the fibrous cap thickness measured by histology using linear regression analyses and paired t-tests. For all analyses, a p-value was considered statistically significant. 3.Results3.1.Spatio-Temporal Laser Speckle AnalysisTwenty of the 118 atherosclerotic plaques that were imaged using LSI were histologically classified as NCFAs. One of the 20 NCFAs showed significant tearing of the fibrous cap in the histological section and was not used in the analyses. For the remaining 19 NCFAs analyzed in our study, the fibrous cap thicknesses measured by histology ranged from 85 to . The average coefficient of variation given by the standard deviation in cap thickness measured between the fiducial marks expressed as a percentage of the mean was 26%. This implies that cap thickness measurements varied on average by 26% between the fiducials marks for the NCFAs analyzed in this study. Figure 3 shows examples in which the speckle decorrelation time constant is plotted as a function of distance from the illumination location for two NCFAs. In these plots, we see that is approximately equal to close to the illumination spot and drops with increasing distance as the speckle pattern transitions to a more rapidly fluctuating pattern due to photon scattering in the necrotic core layer. In Fig. 3, the distance corresponding to 65% of is shown. The distances were for the NCFA with a histologically measured average cap thickness of , and for an average cap thickness of . Fig. 3The figure shows plotted versus distance from the source entry point for two NCFAs with different cap thicknesses. The distance at which drops to 65% of its maximum value is shown, which is related to the cap thickness. ![]() Figure 4 depicts one example of a 2-D colormap illustrating the spatial distribution of measured by translating a correlation window at intervals across a region of a necrotic core fibroatheroma. The dotted line in the colormap demarcates the line along which the corresponding histological section was obtained. In the colormap, is high at the center of the plaque corresponding to a thicker region of the fibrous cap, and rapidly drops as the cap thins out toward the peripheral regions of the NCFA. Fig. 4The colormap shows the spatial distribution of speckle decorrelation time constants measured across a region of a NCFA. The corresponding histological section obtained across the dotted line is shown in the figure, with the fibrous cap demarcated by the solid black line. A high decorrelation time constant is measured at the center of the colormap corresponding to a thicker portion of the fibrous cap at the center of the NCFA, which rapidly drops at the periphery of the colormap where the fibrous cap is very thin. ![]() 3.2.Laser Speckle Imaging-Monte Carlo (LSI-MC) AnalysisFigure 5a shows one example of the radial photon probabilities, , measured experimentally for a fibrous plaque compared with the theoretical radial photon probabilities calculated from the diffusion model for a semi-infinite homogeneous tissue.20 The average optical properties that we obtained by utilizing a least-squares optimization procedure to fit the theoretical curve to the experimental data yielded, for the fibrous plaques: , , and ; and for the normal aortic samples: , , and . These values correspond with previously published results of optical properties of the human aorta at 632-nm wavelength.21 Figure 5b shows the results of the Monte Carlo simulations in which the average radially resolved photon penetration depth , measured for six fibrous plaques, is plotted as a function of distance from the illumination location. The error bars show the standard deviation. The plot shows that the uncertainty in estimating increases with distance from the illumination location. Fig. 5(a) Photon probability, measured from time-averaged speckle images of a fibrous plaque, is plotted as a function of distance from the illumination location. Optical properties of the fibrous plaque were extracted by using a least-squares optimization procedure to fit the theoretical model to experimental data. The measured optical properties were: ; ; and for this fibrous plaque. (b) The maximum photon penetration depth , obtained from the Monte Carlo simulations using optical properties of six fibrous plaques, is plotted as a function of . ![]() Linear regression analysis demonstrated good correlation between and fibrous cap thickness measured from histology at evaluated over the entire range of inflection thresholds of . In Fig. 6 , , where is the radial distance at 65% of , is plotted versus the average fibrous cap thickness measured from histology. Within the threshold range, the parameter at corresponding to 65% of showed the strongest positive correlation with histological measurements . In addition at the 65% inflection threshold, LSI-MC and histological measurements of cap thickness showed the lowest difference in mean measurements between the groups. The results of the paired t-tests showed that the least statistically significant difference between LSI-MC measurements of and histological measurements of fibrous cap thickness for measured at 65% of . Fig. 6The parameter , measured using the LSI-MC technique for corresponding to 65% , is plotted against fibrous cap thickness measured from histology. The solid line depicts the linear least-squares fit through the data, and the dotted lines show the 95% prediction limits. A high correlation was observed between and NCFA fibrous cap thicknesses. ![]() 4.DiscussionIn this work, we describe a new technique to obtain depth-resolved structural information about necrotic-core fibroatheromas by the spatio-temporal analysis of laser speckle patterns. This study demonstrates that by combining analyses of the second-order statistics of time-varying laser speckle fluctuations with diffusion theory and Monte Carlo models of light propagation in tissue, it may be possible to obtain an estimate of fibrous cap thickness in necrotic-core fibroatheromas, which is an important predictor of plaque vulnerability. Due to the differences in viscoelastic properties of the fibrous cap layer and the necrotic core in a NCFA, the Brownian motion of particles within the two layers elicits differences in speckle intensity fluctuations resulting from photon scattering in each layer. By analyzing the spatial variation in the speckle pattern decorrelation time constant as a function of distance , from the source, we obtained the distance at which dropped to 65% of its maximum value. Monte Carlo simulations enabled the determination of the maximum photon penetration depth as a function of radial distance from the source location. When was evaluated at (from LSI), we obtained the parameter that was highly correlated to the average fibrous cap thickness measured from histological sections . The results of the paired t-test demonstrated that the measurements of were not significantly different from histological measurements, suggesting that LSI may provide a viable technique for the estimation of fibrous cap thickness in NCFAs. By analyzing the spatial dependence of , we have demonstrated that 2-D maps can be reconstructed to evaluate the variation in fibrous cap thickness across the NCFA (Fig. 4). Using these methods, it may be possible to illustrate regions of focal thinning within the fibrous cap to aid in identifying potential sites for plaque rupture. When combined with illumination beam scanning, this feature of LSI could enable the acquisition of 3-D volumetric maps of plaque morphology and viscoelasticity distributions. In this work, the diffusion theory model of spatially resolved diffusive reflectance developed by Farell, Patterson, and Wilson was used for optical characterization of the aortic tissues.20 By fitting the theoretical prediction of radially resolved photon probability to experimentally measured data, we extracted the optical properties for aortic fibrous plaques. In our study, the experimental measurements of photon probabilities were normalized with the theoretical curves by including a scaling factor. The scaling factor, determined using a least-squares fitting procedure, was included to account for imaging system parameters such as the limited solid angle of collection due to the finite aperture of the imaging lens system, the angle of the CCD camera with respect to the tissue surface, and the sensitivity of the CCD camera. This study has shown that it is possible to characterize optical properties of tissue from analyses of laser speckle images with relative experimental ease. The technique thus lends itself to the possibility of optically characterizing different types of atherosclerotic plaques for a variety of research as well as clinical applications. Although the LSI-MC technique shows good correlation with histological measurements of cap thickness, and there is no significant difference between these groups, the factors that may influence the precision of LSI-MC measurements are: 1. the distance beyond which speckle fluctuations are predominantly due to light scattering from the necrotic core; 2. the optical properties of the fibrous cap, which defines the accuracy of the MC calibration curve; and 3. the measurement of the average fibrous cap thickness in histological sections. The influence of these factors on LSI-MC measurements is described next. As described before, was determined as the distance from the illumination location at which dropped to a given percent of its maximum value . In our experiments, we evaluated the parameter for corresponding to different thresholds of . The endpoints of the threshold range were selected based on our empirical results. Within the threshold range of 80 to 40% , differences between LSI-MC measurements of were not statistically significant. At the endpoints of the range, i.e., at 80 and 40% of , LSI-MC measurements were significantly different from histological measurements (at and 40% ). Based on these findings, we limited our range of thresholds between 80 to 40% . We found that LSI-MC measurements of evaluated for at 65% of showed the strongest correlation and the smallest statistically significant difference with histological measurements. From these results, we may infer that evaluated at 65% of provided the best approximation of the inflection distance. However, the true inflection distance at which the speckle pattern transitions from the fibrous cap to the necrotic core may in fact be less than the measured inflection distance . The size of the cross-correlation window used to calculate the radially resolved value of influences the determination of the true inflection distance, due to the blurring effect of the finite window on the data. Hence, the measured does not show a sharp drop at the inflection point, and instead, a smooth roll off of is often observed as a function of (Fig. 3). In our study, the window size was selected empirically. The optimum size of the cross-correlation window may be influenced by the statistical uncertainty in detecting photons returning from depth, remitted within the finite window centered at . With future studies, it may be possible to optimize the size of the cross-correlation window by applying the results of MC simulations of radially resolved photon penetration depths to minimize the statistical uncertainty in detecting photons remitted within a finite window. Thus, by optimizing the size of the cross-correlation window, it may be possible to obtain increased spatial resolution in measuring , and the inflection distance may be determined more precisely. The diffusion theory and Monte Carlo models in this study characterize only the fibrous cap of the NCFA, by describing a single-layered, semi-infinite, homogeneous medium. A more accurate representation of the NCFA would involve a two-layered model with different sets of optical properties to describe the fibrous cap and the necrotic core. However, the measurement of optical properties of the necrotic core can be a challenging task, as the core can be extremely heterogeneous in composition, consisting of lipid, collagen fibrils, cholesterol crystals, inflammatory cells, and necrotic debris. Furthermore, the optical properties of the necrotic core may vary considerably across plaques and across patients, and as a result may be difficult to characterize. In our analyses, we utilize a spatio-temporal LSI technique to determine the distance . We can approximate that photons remitted at distances greater than have exited through the fibrous cap and propagated into the necrotic core. Likewise, photons remitted at distances less than have predominantly traversed only through the fibrous cap. Since the fibrous cap can be assumed as a single-layered homogeneous medium, and by measuring the maximum photon penetration depth , only through this fibrous medium constrained by a distance from the source, we utilized a simplified model of the fibrous cap and did not require characterizing the optical properties of the necrotic core. For ease of implementation, we only measured optical properties using six collagen-rich fibrous plaques in the present study. Although we have not investigated the intra-plaque and intra-patient differences in the optical properties of the aortic fibrous tissue, we expect that these differences may be negligible, as the fibrous cap predominantly consists of collagen. However, for successful in vivo application, it may be important to develop a fibrous cap model by analyzing several fibrous plaques, to investigate the variation of optical parameters due to fibrous cap constituents such as collagen types 1 and 3, the presence of smooth muscle cells, and macrophages. Further investigation of such a model would reduce the statistical uncertainty in determining the optical properties of the fibrous cap, thereby enabling future spatio-temporal LSI measurements of fibrous cap thickness with increased precision. In addition, we observed that the optical properties of fibrous plaques did not differ significantly from those measured from normal aortic tissue samples, in spite of possible differences in the compositions of the two tissue types. In a fibrous plaque, laser speckle images would mainly be influenced by light scattering within an extensively thickened intimal layer predominantly consisting of collagen. However, the adult aorta, which may appear normal on gross examination, often shows intimal hyperplasia (thickening of the intima up to ) with increased deposition of collagen and smooth muscle cell proliferation in the intima. Due to intimal hyperplasia, laser speckle fluctuations may similarly be influenced by scattering within the thick intimal as well as the medial layers. As a result, optical properties measured from time-averaged speckle images of the fibrous plaque and normal aorta may not be significantly different in our study. Registration of speckle images with the corresponding histology was performed using ink marks on the lesion site. Since fibrous cap thickness may vary as a function of measurement location, registration errors between speckle and histology images could affect LSI results. Since cadaveric specimens were used in the study and were stored in PBS for up to before imaging, minor degradation of the specimen may have occurred. The effect of tissue degradation on the optical properties of plaques was not tested in this study. However, previous experience with other optical imaging techniques in our laboratory suggest that the optical properties of vascular tissue undergo only minor changes as long as the measurements are performed within of autopsy. In our study, although speckle images were acquired over a 2-s duration, measurements were performed by single exponential fitting of the region of the normalized speckle decorrelation curve, over which the cross-correlation value dropped to 75% of its maximum, corresponding to an average acquisition time of less than . For future in vivo applications, this relatively short acquisition time requirement would allow for a sufficient temporal window during the resting phase of the cardiac cycle to obtain diagnostic quality speckle data. In addition, a CCD camera with a significantly higher image acquisition rate could be used to provide higher temporal resolution of time-varying speckle data, which may allow for measurements of speckle decorrelation to be performed over a few milliseconds. We have already demonstrated in our previous study that LSI measurements can be obtained with high accuracy even under physiological rates of maximal arterial deformation.11 LSI may be extended to patient studies by using small diameter flexible optical fiber bundles, similar to those of coronary angioscopy, to obtain laser speckle images of coronary plaques.22, 23 Intracoronary saline flushing, which has been successfully implemented in in vivo optical coherence tomography and angioscopy procedures to temporarily displace blood by injecting a bolus of saline, can be utilized in conjunction with LSI to enable unobstructed imaging of the coronary wall.24, 25 LSI is a unique technique, as it enables plaque characterization by providing measurements that are related to the viscoelastic properties of atherosclerotic lesions. It has been previously demonstrated that at a single beam location, LSI can identify plaque type and measure an index of viscoelasticity that is related to plaque composition. In this study, we have demonstrated the ability of LSI to provide depth-resolved information. By combining LSI measurements of the spatial variation in decorrelation time constants with a Monte Carlo model to describe light propagation through the plaque, it is possible to measure a parameter that is highly correlated with fibrous cap thickness, which is a critical anatomical predictor of plaque rupture. Based on its unique ability to provide both biomechanical and structural information, we anticipate that catheter-based LSI will become a powerful tool for detecting high risk lesions to target local therapy prior to the onset of an acute coronary event. 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