Collagen birefringence in skin repair in response to red polarized-laser therapy

Daniela de Fátima Teixeira da Silva; Benedicto de Campos Vidal; Denise Maria Zezell; Telma M. T. Zorn; Silvia Cristina Núñez; Martha S. Ribeiro

doi:10.1117/1.2187418

1 March 2006 Collagen birefringence in skin repair in response to red polarized-laser therapy

Daniela de Fátima Teixeira da Silva, Benedicto de Campos Vidal, Denise Maria Zezell, Telma M. T. Zorn, Silvia Cristina Núñez, Martha S. Ribeiro

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Journal of Biomedical Optics, Vol. 11, Issue 2, 024002 (March 2006). https://doi.org/10.1117/1.2187418

Abstract

We use the optical path difference (OPD) technique to quantify the organization of collagen fibers during skin repair of full-thickness burns following low-intensity polarized laser therapy with two different polarization incidence vectors. Three burns are cryogenerated on the back of rats. Lesion L_|| is irradiated using the electric field vector of the polarized laser radiation aligned in parallel with the rat's occipital-caudal direction. Lesion L_⊥ is irradiated using the electric field vector of the polarized laser radiation aligned perpendicularly to the aforementioned orientation. Lesion C is untreated. A healthy area labeled H is also evaluated. The tissue samples are collected and processed for polarized light microscopy. The overall finding is that the OPD for collagen fibers depends on the electric field vector of the incident polarized laser radiation. No significant differences in OPDs are observed between L_|| and H in the center, sides, and edges of the lesion. Lesions irradiated using the electric field vector of the polarized laser radiation aligned in parallel with the rat's occipital-caudal direction show higher birefringence, indicating that collagen bundles in these lesions are more organized.

©(2006) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Daniela de Fátima Teixeira da Silva, Benedicto de Campos Vidal, Denise Maria Zezell, Telma M. T. Zorn, Silvia Cristina Núñez, and Martha S. Ribeiro "Collagen birefringence in skin repair in response to red polarized-laser therapy," Journal of Biomedical Optics 11(2), 024002 (1 March 2006). https://doi.org/10.1117/1.2187418

Published: 1 March 2006

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