Use of light absorbers to alter optical interrogation with epi-illumination and transillumination in three-dimensional cardiac models

Venkat K. Ramshesh; Stephen B. Knisley

doi:10.1117/1.2187012

1 March 2006 Use of light absorbers to alter optical interrogation with epi-illumination and transillumination in three-dimensional cardiac models

Venkat K. Ramshesh, Stephen B. Knisley

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Journal of Biomedical Optics, Vol. 11, Issue 2, 024019 (March 2006). https://doi.org/10.1117/1.2187012

Abstract

Cardiac optical mapping currently provides 2-D maps of transmembrane voltage-sensitive fluorescence localized near the tissue surface. Methods for interrogation at different depths are required for studies of arrhythmias and the effects of defibrillation shocks in 3-D cardiac tissue. We model the effects of coloading with a dye that absorbs excitation or fluorescence light on the radius and depth of the interrogated region with specific illumination and collection techniques. Results indicate radii and depths of interrogation are larger for transillumination versus epi-illumination, an effect that is more pronounced for broad-field excitation versus laser scanner. Coloading with a fluorescence absorber lessens interrogated depth for epi-illumination and increases it for transillumination, which is confirmed with measurements using transillumination of heart tissue slices. Coloading with an absorber of excitation light consistently decreases the interrogated depths. Transillumination and coloading also decrease the intensities of collected fluorescence. Thus, localization can be modified with wavelength-specific absorbers at the expense of a reduction in fluorescence intensity.

©(2006) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Venkat K. Ramshesh and Stephen B. Knisley "Use of light absorbers to alter optical interrogation with epi-illumination and transillumination in three-dimensional cardiac models," Journal of Biomedical Optics 11(2), 024019 (1 March 2006). https://doi.org/10.1117/1.2187012

Published: 1 March 2006

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KEYWORDS

Luminescence

Tissue optics

Photons

Absorption

Natural surfaces

Tissues

Laser scanners

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