Dehydration mechanism of optical clearing in tissue

Christopher G. Rylander; Oliver F. Stumpp; Thomas E. Milner; Nathaniel Joseph Kemp; John M. Mendenhall; Kenneth R. Diller; Ashley J. Welch

doi:10.1117/1.2343208

1 July 2006 Dehydration mechanism of optical clearing in tissue

Christopher G. Rylander, Oliver F. Stumpp, Thomas E. Milner, Nathaniel Joseph Kemp, John M. Mendenhall, Kenneth R. Diller, Ashley J. Welch

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Journal of Biomedical Optics, Vol. 11, Issue 4, 041117 (July 2006). https://doi.org/10.1117/1.2343208

Abstract

Previous studies identified various mechanisms of light scattering reduction in tissue induced by chemical agents. Our results suggest that dehydration is an important mechanism of optical clearing in collagenous and cellular tissue. Photographic and optical coherence tomography images indicate that air-immersed skin and tendon specimens become similarly transparent to glycerol-immersed specimens. Transmission electron microscopy images reveal that dehydration causes individual scattering particles such as collagen fibrils and organelles to become more densely packed, but does not significantly alter size. A heuristic particle-interaction model predicts that the scattering particle volume fraction increase can contribute substantially to optical clearing in collagenous and cellular tissue.

©(2006) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Christopher G. Rylander, Oliver F. Stumpp, Thomas E. Milner, Nathaniel Joseph Kemp, John M. Mendenhall, Kenneth R. Diller, and Ashley J. Welch "Dehydration mechanism of optical clearing in tissue," Journal of Biomedical Optics 11(4), 041117 (1 July 2006). https://doi.org/10.1117/1.2343208

Published: 1 July 2006

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KEYWORDS

Scattering

Tissue optics

Optical clearing

Light scattering

Particles

Tissues

Refractive index

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