Rab4 and Rab11 coordinately regulate the recycling of angiotensin II type I receptor as demonstrated by fluorescence resonance energy transfer microscopy

Hewang Li; Hui-Fang Li; Robin A. Felder; Ammasi Periasamy; Pedro A. Jose

doi:10.1117/1.2943286

1 May 2008 Rab4 and Rab11 coordinately regulate the recycling of angiotensin II type I receptor as demonstrated by fluorescence resonance energy transfer microscopy

Hewang Li, Hui-Fang Li, Robin A. Felder, Ammasi Periasamy, Pedro A. Jose

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Journal of Biomedical Optics, Vol. 13, Issue 3, 031206 (May 2008). https://doi.org/10.1117/1.2943286

Abstract

The recycling of G-protein-coupled receptors (GPCR) to the cell surface after internalization plays an important role in the regulation of overall GPCR activity. The angiotensin II type I receptor (AT₁R) belongs to class B GPCRs that recycle slowly back to the cell surface. Previous studies have proposed that Rab11 controls the recycling of AT₁R; however, recent reports show that Rab4, a rapid recycling regulator, co-localizes also with internalized AT₁R. Different from the subcellular co-localization provided by fluorescence microscopy, fluorescence resonance energy transfer (FRET) microscopy provided the spatial relationship of AT₁R with Rab4 and Rab11 in the nanometer-range proximity during the entire course of AT₁R recycling. During the early recycling stage, internalized AT₁Rs were mainly associated with Rab4 in the cytoplasm. During the mid-recycling stage, AT₁Rs were associated with both Rab4 and Rab11 in the perinuclear compartments. However, during the late-recycling stage, AT₁Rs were mainly associated with Rab11, both in the perinuclear compartments and the plasma membrane. Co-immunoprecipitation data confirmed these dynamic associations, which were disrupted by silencing of either the Rab4 or Rab11 gene. Based on these observations, we propose a Rab4 and Rab11 coordinated model for AT₁R recycling.

©(2008) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Hewang Li, Hui-Fang Li, Robin A. Felder, Ammasi Periasamy, and Pedro A. Jose "Rab4 and Rab11 coordinately regulate the recycling of angiotensin II type I receptor as demonstrated by fluorescence resonance energy transfer microscopy," Journal of Biomedical Optics 13(3), 031206 (1 May 2008). https://doi.org/10.1117/1.2943286

Published: 1 May 2008

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