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1 January 2010 Cellular resolution ex vivo imaging of gastrointestinal tissues with optical coherence microscopy
Aaron D. Aguirre, Yu Chen, Bradley Bryan M.D., Hiroshi Mashimo, Qin Huang, James L. Connolly, James G. Fujimoto
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Journal of Biomedical Optics, Vol. 15, Issue 1, 016025 (January 2010). https://doi.org/10.1117/1.3322704
Abstract
Optical coherence microscopy (OCM) combines confocal microscopy and optical coherence tomography (OCT) to improve imaging depth and contrast, enabling cellular imaging in human tissues. We aim to investigate OCM for ex vivo imaging of upper and lower gastrointestinal tract tissues, to establish correlations between OCM imaging and histology, and to provide a baseline for future endoscopic studies. Co-registered OCM and OCT imaging were performed on fresh surgical specimens and endoscopic biopsy specimens, and images were correlated with histology. Imaging was performed at 1.06-µm wavelength with <2-μm transverse and <4-μm axial resolution for OCM, and at 14-μm transverse and <3-μm axial resolution for OCT. Multiple sites on 75 tissue samples from 39 patients were imaged. OCM enabled cellular imaging of specimens from the upper and lower gastrointestinal tracts over a smaller field of view compared to OCT. Squamous cells and their nuclei, goblet cells in Barrett's esophagus, gastric pits and colonic crypts, and fine structures in adenocarcinomas were visualized. OCT provided complementary information through assessment of tissue architectural features over a larger field of view. OCM may provide a complementary imaging modality to standard OCT approaches for endoscopic microscopy.
©(2010) Society of Photo-Optical Instrumentation Engineers (SPIE)
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Aaron D. Aguirre, Yu Chen, Bradley Bryan M.D., Hiroshi Mashimo, Qin Huang, James L. Connolly, and James G. Fujimoto "Cellular resolution ex vivo imaging of gastrointestinal tissues with optical coherence microscopy," Journal of Biomedical Optics 15(1), 016025 (1 January 2010). https://doi.org/10.1117/1.3322704
Published: 1 January 2010
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Cited by 43 scholarly publications and 1 patent.
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KEYWORDS
Optical coherence tomography
Tissues
Confocal microscopy
Endoscopy
Image resolution
Imaging systems
Visualization
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