Three-dimensional Fourier domain optical coherence tomography in vivo imaging of alveolar tissue in the intact thorax using the parietal pleura as a window

Sven Meissner; Lilla Knels; Christian Schnabel; Thea Koch; Edmund Koch

doi:10.1117/1.3302809

1 January 2010 Three-dimensional Fourier domain optical coherence tomography in vivo imaging of alveolar tissue in the intact thorax using the parietal pleura as a window

Sven Meissner, Lilla Knels, Christian Schnabel, Thea Koch, Edmund Koch

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Journal of Biomedical Optics, Vol. 15, Issue 1, 016030 (January 2010). https://doi.org/10.1117/1.3302809

Abstract

In vivo determination of 3-D and dynamic geometries of alveolar structures with adequate resolution is essential for developing numerical models of the lung. A thorax window is prepared in anesthetized rabbits by removal of muscle tissue between the third and fourth rib without harming the parietal pleura. The transparent parietal pleura allows contact-free imaging by intravital microscopy (IVM) and 3-D optical coherence tomography (3-D OCT). We demonstrate that dislocation of the lung surface is small enough to observe identical regions in the expiratory and inspiratory plateau phase, and that OCT in this animal model is suitable for generating 3-D geometry of in vivo lung parenchyma. To our knowledge, we present a novel thorax window preparation technique for 3-D imaging of alveolar dynamics for the first time. The 3-D datasets of the fine structure of the lung beneath the pleura could provide a basis for the development of 3-D numerical models of the lung.

©(2010) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Sven Meissner, Lilla Knels, Christian Schnabel, Thea Koch, and Edmund Koch "Three-dimensional Fourier domain optical coherence tomography in vivo imaging of alveolar tissue in the intact thorax using the parietal pleura as a window," Journal of Biomedical Optics 15(1), 016030 (1 January 2010). https://doi.org/10.1117/1.3302809

Published: 1 January 2010

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