Shack-Hartmann wavefront-sensor-based adaptive optics system for multiphoton microscopy

Jae-Won Cha; Jérôme Ballesta; Peter T. C. So

doi:10.1117/1.3475954

1 July 2010 Shack-Hartmann wavefront-sensor-based adaptive optics system for multiphoton microscopy

Jae-Won Cha, Jérôme Ballesta, Peter T. C. So

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Journal of Biomedical Optics, Vol. 15, Issue 4, 046022 (July 2010). https://doi.org/10.1117/1.3475954

Abstract

The imaging depth of two-photon excitation fluorescence microscopy is partly limited by the inhomogeneity of the refractive index in biological specimens. This inhomogeneity results in a distortion of the wavefront of the excitation light. This wavefront distortion results in image resolution degradation and lower signal level. Using an adaptive optics system consisting of a Shack-Hartmann wavefront sensor and a deformable mirror, wavefront distortion can be measured and corrected. With adaptive optics compensation, we demonstrate that the resolution and signal level can be better preserved at greater imaging depth in a variety of ex-vivo tissue specimens including mouse tongue muscle, heart muscle, and brain. However, for these highly scattering tissues, we find signal degradation due to scattering to be a more dominant factor than aberration.

©(2010) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Jae-Won Cha, Jérôme Ballesta, and Peter T. C. So "Shack-Hartmann wavefront-sensor-based adaptive optics system for multiphoton microscopy," Journal of Biomedical Optics 15(4), 046022 (1 July 2010). https://doi.org/10.1117/1.3475954

Published: 1 July 2010

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