Open Access
1 September 2010 Digital holographic microscopy by use of surface plasmon resonance for imaging of cell membranes
Cuiying Hu, Jingang Zhong, Jiawen Weng
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Abstract
A technique called surface plasmon resonance digital holographic microscopy (SPRDHM) for optical imaging of cell membranes is proposed. The intensity and phase distributions of the reflected light that is modulated by the cell membrane in surface plasmon resonance can be simultaneously obtained. The imaging principle and capability are theoretically analyzed and demonstrated by experiments. In addition, the technique is compared with total internal reflection digital holographic microscopy (TIRDHM) in theory and experiment, respectively. The results show that the SPRDHM technique is better in spatial resolution and phase sensitivity than the TIRDHM technique for imaging of cell membranes
©(2010) Society of Photo-Optical Instrumentation Engineers (SPIE)
Cuiying Hu, Jingang Zhong, and Jiawen Weng "Digital holographic microscopy by use of surface plasmon resonance for imaging of cell membranes," Journal of Biomedical Optics 15(5), 056015 (1 September 2010). https://doi.org/10.1117/1.3497564
Published: 1 September 2010
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CITATIONS
Cited by 18 scholarly publications.
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KEYWORDS
Digital holography

Refractive index

Surface plasmons

3D image reconstruction

Holography

Microscopy

Gold

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