Rapid confocal imaging of large areas of excised tissue with strip mosaicing

Sanjeewa Abeytunge; Yongbiao Li; Ricardo Toledo-Crow; Bjorg A. Larson; Milind Rajadhyaksha

doi:10.1117/1.3582335

1 May 2011 Rapid confocal imaging of large areas of excised tissue with strip mosaicing

Sanjeewa Abeytunge, Yongbiao Li, Ricardo Toledo-Crow, Bjorg A. Larson, Milind Rajadhyaksha

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Journal of Biomedical Optics, Vol. 16, Issue 5, 050504 (May 2011). https://doi.org/10.1117/1.3582335

Abstract

Imaging large areas of tissue rapidly and with high resolution may enable rapid pathology at the bedside. The limited field of view of high-resolution microscopes requires the merging of multiple images that are taken sequentially to cover a large area. This merging or mosaicing of images requires long acquisition and processing times, and produces artifacts. To reduce both time and artifacts, we developed a mosaicing method on a confocal microscope that images morphology in large areas of excised tissue with sub-cellular detail. By acquiring image strips with aspect ratios of 10:1 and higher (instead of the standard ∼1:1) and "stitching" them in software, our method images 10×10 mm2 area of tissue in about 3 min. This method, which we call "strip mosaicing," is currently three times as fast as our previous method.

©(2011) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Sanjeewa Abeytunge, Yongbiao Li, Ricardo Toledo-Crow, Bjorg A. Larson, and Milind Rajadhyaksha "Rapid confocal imaging of large areas of excised tissue with strip mosaicing," Journal of Biomedical Optics 16(5), 050504 (1 May 2011). https://doi.org/10.1117/1.3582335

Published: 1 May 2011

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