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12 March 2012 Targeted detection of murine colonic dysplasia in vivo with flexible multispectral scanning fiber endoscopy
Sharon J. Miller, Bishnu Joshi, Thomas Duen-Shyr Wang, Cameron M. Lee, Eric J. Seibel, Adam Gaustad
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Journal of Biomedical Optics, Vol. 17, Issue 2, 021103 (March 2012). https://doi.org/10.1117/1.JBO.17.2.021103
Abstract
Gastrointestinal cancers are heterogeneous and can overexpress several protein targets that can be imaged simultaneously on endoscopy using multiple molecular probes. We aim to demonstrate a multispectral scanning fiber endoscope for wide-field fluorescence detection of colonic dysplasia. Excitation at 440, 532, and 635 nm is delivered into a single spiral scanning fiber, and fluorescence is collected by a ring of light-collecting optical fibers placed around the instrument periphery. Specific-binding peptides are selected with phage display technology using the CPC;Apc mouse model of spontaneous colonic dysplasia. Validation of peptide specificity is performed on flow cytometry and in vivo endoscopy. The peptides KCCFPAQ, AKPGYLS, and LTTHYKL are selected and labeled with 7-diethylaminocoumarin-3-carboxylic acid (DEAC), 5-carboxytetramethylrhodamine (TAMRA), and CF633, respectively. Separate droplets of KCCFPAQ-DEAC, AKPGYLS-TAMRA, and LTTHYKL-CF633 are distinguished at concentrations of 100 and 1 μM. Separate application of the fluorescent-labeled peptides demonstrate specific binding to colonic adenomas. The average target/background ratios are 1.71±0.19 and 1.67±0.12 for KCCFPAQ-DEAC and AKPGYLS-TAMRA, respectively. Administration of these two peptides together results in distinct binding patterns in the blue and green channels. Specific binding of two or more peptides can be distinguished in vivo using a novel multispectral endoscope to localize colonic dysplasia on real-time wide-field imaging.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2012/$25.00 © 2012 SPIE
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Sharon J. Miller, Bishnu Joshi, Thomas Duen-Shyr Wang, Cameron M. Lee, Eric J. Seibel, and Adam Gaustad "Targeted detection of murine colonic dysplasia in vivo with flexible multispectral scanning fiber endoscopy," Journal of Biomedical Optics 17(2), 021103 (12 March 2012). https://doi.org/10.1117/1.JBO.17.2.021103
Published: 12 March 2012
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KEYWORDS
In vivo imaging
Endoscopes
Target detection
Luminescence
Endoscopy
Colon
Tissues
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