CONFERENCE PROCEEDINGS
Advanced Search
Home > Journals > J. Biomed. Opt. > Volume 17 > Issue 9 > Article
Open Access
6 September 2012 Sensing dynamic cytoplasm refractive index changes of adherent cells with quantitative phase microscopy using incorporated microspheres as optical probes
Sabine Przibilla, Sebastian Dartmann, Angelika Vollmer, Steffi Ketelhut, Gert von Bally, Björn Kemper, Burkhard Greve
Author Affiliations +
Journal of Biomedical Optics, Vol. 17, Issue 9, 097001 (September 2012). https://doi.org/10.1117/1.JBO.17.9.097001
Abstract
The intracellular refractive index is an important parameter that describes the optical density of the cytoplasm and the concentration of the intracellular solutes. The refractive index of adherently grown cells is difficult to access. We present a method in which silica microspheres in living cells are used to determine the cytoplasm refractive index with quantitative phase microscopy. The reliability of our approach for refractive index retrieval is shown by data from a comparative study on osmotically stimulated adherent and suspended human pancreatic tumor cells. Results from adherent human fibro sarcoma cells demonstrate the capability of the method for sensing of dynamic refractive index changes and its usage with microfluidics.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2012/$25.00 © 2012 SPIE
Citation Download Citation
Sabine Przibilla, Sebastian Dartmann, Angelika Vollmer, Steffi Ketelhut, Gert von Bally, Björn Kemper, and Burkhard Greve "Sensing dynamic cytoplasm refractive index changes of adherent cells with quantitative phase microscopy using incorporated microspheres as optical probes," Journal of Biomedical Optics 17(9), 097001 (6 September 2012). https://doi.org/10.1117/1.JBO.17.9.097001
Published: 6 September 2012
JOURNAL ARTICLE
 9 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Lens.org Logo
CITATIONS
Cited by 37 scholarly publications.
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Refractive index
Digital holography
Particles
Microscopy
Phase contrast
Holograms
Microfluidics
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top