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6 February 2013 Applying tattoo dye as a third-harmonic generation contrast agent for in vivo optical virtual biopsy of human skin
Ming-Rung Tsai, Chen-Yu Lin, Yi-Hua Liao M.D., Chi-Kuang Sun
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Journal of Biomedical Optics, Vol. 18, Issue 2, 026012 (February 2013). https://doi.org/10.1117/1.JBO.18.2.026012
Abstract
Third-harmonic generation (THG) microscopy has been reported to provide intrinsic contrast in elastic fibers, cytoplasmic membrane, nucleus, actin filaments, lipid bodies, hemoglobin, and melanin in human skin. For advanced molecular imaging, exogenous contrast agents are developed for a higher structural or molecular specificity. We demonstrate the potential of the commonly adopted tattoo dye as a THG contrast agent for in vivo optical biopsy of human skin. Spectroscopy and microscopy experiments were performed on cultured cells with tattoo dyes, in tattooed mouse skin, and in tattooed human skin to demonstrate the THG enhancement effect. Compared with other absorbing dyes or nanoparticles used as exogenous THG contrast agents, tattoo dyes are widely adopted in human skin so that future clinical biocompatibility evaluation is relatively achievable. Combined with the demonstrated THG enhancement effect, tattoo dyes show their promise for future clinical imaging applications.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Ming-Rung Tsai, Chen-Yu Lin, Yi-Hua Liao M.D., and Chi-Kuang Sun "Applying tattoo dye as a third-harmonic generation contrast agent for in vivo optical virtual biopsy of human skin," Journal of Biomedical Optics 18(2), 026012 (6 February 2013). https://doi.org/10.1117/1.JBO.18.2.026012
Published: 6 February 2013
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Cited by 8 scholarly publications and 1 patent.
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KEYWORDS
Skin
In vivo imaging
Microscopy
Third-harmonic generation
Biopsy
Luminescence
Nanoparticles
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