Staining-free malaria diagnostics by multispectral and multimodality light-emitting-diode microscopy

Aboma J. Merdasa; Mikkel Brydegaard; Sune Svanberg; Jeremie T. Zoueu

doi:10.1117/1.JBO.18.3.036002

1 March 2013 Staining-free malaria diagnostics by multispectral and multimodality light-emitting-diode microscopy

Aboma J. Merdasa, Mikkel Brydegaard, Sune Svanberg, Jeremie T. Zoueu

Author Affiliations +

Journal of Biomedical Optics, Vol. 18, Issue 3, 036002 (March 2013). https://doi.org/10.1117/1.JBO.18.3.036002

Abstract

We report an accurate optical differentiation technique between healthy and malaria-infected erythrocytes by quasi-simultaneous measurements of transmittance, reflectance, and scattering properties of unstained blood smears using a multispectral and multimode light-emitting diode microscope. We propose a technique for automated imaging, identification, and counting of malaria-infected erythrocytes for real-time and cost-effective parasitaemia diagnosis as an effective alternative to the manual screening of stained blood smears, now considered to be the gold standard in malaria diagnosis. We evaluate the performance of our algorithm against manual estimations of an expert and show a spectrally resolved increased scattering from malaria-infected blood cells.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

Citation Download Citation

Aboma J. Merdasa, Mikkel Brydegaard, Sune Svanberg, and Jeremie T. Zoueu "Staining-free malaria diagnostics by multispectral and multimodality light-emitting-diode microscopy," Journal of Biomedical Optics 18(3), 036002 (1 March 2013). https://doi.org/10.1117/1.JBO.18.3.036002

Published: 1 March 2013

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
11 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

CITATIONS

Cited by 18 scholarly publications.

Explore citations on Lens.org

KEYWORDS

Scattering

Blood

Light scattering

Reflectivity

Absorption

Microscopes

Diagnostics

Show All Keywords

RELATED CONTENT

Simulation of light transport in arthritic and non arthritic...
Proceedings of SPIE (March 04 2014)

Cell classification using big data analytics plus time stretch imaging (Conference...
Proceedings of SPIE (January 01 1900)

Effects of compression on human skin optical properties
Proceedings of SPIE (August 18 1997)

PC-LED luminance enhancement due to phosphor scattering
Proceedings of SPIE (August 22 2005)

Differences in thermal optical response between intact diabetic and nondiabetic...
Proceedings of SPIE (July 22 2003)

Determination of optical constants and morphological parameters of biological particles...
Proceedings of SPIE (July 21 1993)

Apparent optical density of the scattering medium influence of...
Proceedings of SPIE (July 16 2002)

Subscribe to Digital Library

Receive Erratum Email Alert

Show All Keywords

Keywords/Phrases

Search In:

Publication Years