Evaluation of parameters influencing the molecular delivery by biodegradable microsphere-mediated perforation using femtosecond laser

Tatsuki Mitsuhashi; Mitsuhiro Terakawa

doi:10.1117/1.JBO.19.1.015003

3 January 2014 Evaluation of parameters influencing the molecular delivery by biodegradable microsphere-mediated perforation using femtosecond laser

Tatsuki Mitsuhashi, Mitsuhiro Terakawa

Author Affiliations +

Journal of Biomedical Optics, Vol. 19, Issue 1, 015003 (January 2014). https://doi.org/10.1117/1.JBO.19.1.015003

Abstract

The parameters critically influencing the delivery rate on the biodegradable microsphere-mediated femtosecond (fs) laser perforation are investigated in detail with the aim of developing efficient molecular delivery. Cell membrane was perforated by the irradiation of weakly focused fs laser pulses to the spherical polylactic acid microspheres conjugated to the cell membrane. The delivery of fluorescein isothiocyanate-dextran and fluorescent silica particles to A431 cells is investigated in detail. The increase in the number of irradiated laser pulses had resulted in the increase of delivery rate. The delivery rate depends on the size and functionalization of fluorescent silica particles in which silica particles of 100 nm in diameter were able to be delivered into 20% of the irradiated cells, suggesting that the pore sizes are large enough for the delivery of therapeutic agents into cells. These findings contribute to the development of an efficient and safe phototherapy and drug delivery.

Citation Download Citation

Tatsuki Mitsuhashi and Mitsuhiro Terakawa "Evaluation of parameters influencing the molecular delivery by biodegradable microsphere-mediated perforation using femtosecond laser," Journal of Biomedical Optics 19(1), 015003 (3 January 2014). https://doi.org/10.1117/1.JBO.19.1.015003

Published: 3 January 2014

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY