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27 January 2014 Multiplexed label-free optical biosensor for medical diagnostics
Barbara Bottazzi, Lucia Fornasari, Ana Frangolho, Silvia Giudicatti, Alberto Mantovani, Franco Marabelli, Gerardo Marchesini, Paola Pellacani, Rita Therisod, Andrea Valsesia
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Journal of Biomedical Optics, Vol. 19, Issue 1, 017006 (January 2014). https://doi.org/10.1117/1.JBO.19.1.017006
Abstract
This paper describes a new multiplexed label-free biosensor. The detection technology is based on nanostructured gold-polymer surfaces. These surfaces support surface plasmon resonance modes that can be probed by a miniaturized optical setup. The optical characterization of the sensing chip shows the sensitivity and the limit-of-detection to refractive index changes. Moreover, by studying the progressive adhesion of molecular monolayers of polyelectrolytes, the decay of the plasmonic mode electric field above the surface has been reconstructed. A multiplexed label-free biosensing device is then described and characterized in terms of sensitivity, lateral resolution, and sensitivity to a model biological assay. The sensitivity in imaging mode of the device is of the order of 10 −6 refractive index units, while the measured lateral resolution is 6.25 μm within a field of view of several tenths of mm 2 , making the instrument unique in terms of multiplexing capability. Finally, the proof-of-concept application of the technology as a point-of-care diagnostic tool for an inflammatory marker is demonstrated.
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2014/$25.00 © 2014 SPIE
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Barbara Bottazzi, Lucia Fornasari, Ana Frangolho, Silvia Giudicatti, Alberto Mantovani, Franco Marabelli, Gerardo Marchesini, Paola Pellacani, Rita Therisod, and Andrea Valsesia "Multiplexed label-free optical biosensor for medical diagnostics," Journal of Biomedical Optics 19(1), 017006 (27 January 2014). https://doi.org/10.1117/1.JBO.19.1.017006
Published: 27 January 2014
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KEYWORDS
Refractive index
Reflectivity
Multiplexing
Water
Plasmonics
Molecules
Sensors
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