CONFERENCE PROCEEDINGS
Advanced Search
Home > Journals > J. Biomed. Opt. > Volume 19 > Issue 11 > Article
Open Access
23 July 2014 Microfluidic surface-enhanced Raman scattering sensor with monolithically integrated nanoporous gold disk arrays for rapid and label-free biomolecular detection
Ming Li, Fusheng Zhao, Jianbo Zeng, Ji Qi, Jing Lu, Wei-Chuan Shih
Author Affiliations +
Journal of Biomedical Optics, Vol. 19, Issue 11, 111611 (July 2014). https://doi.org/10.1117/1.JBO.19.11.111611
Abstract
We present a microfluidic surface-enhanced Raman scattering (SERS) sensor for rapid and label-free biomolecular detection. Our sensor design mitigates a common limiting factor in microfluidic SERS sensors that utilize integrated nanostructures: low-efficiency transport of biomolecules to nanostructured surface which adversely impacts sensitivity. Our strategy is to increase the total usable nanostructured surface area, which provides more adsorption sites for biomolecules. Specifically, a nanoporous gold disk (NPGD) array, a highly effective SERS substrate, has been monolithically integrated inside a microfluidic chip. Individual NPGD is known to feature an order of magnitude larger surface area than its projected disk area. The increased surface area arises from nanoscale pores and ligaments three-dimensionally distributed in the NPGD, which manifest themselves as high-density SERS hot-spots. High-density NPGD arrays further guarantee large coverage of these hot-spots on the microchannel floor. The sensor performance has been demonstrated using Rhodamine 6G to quantify spatial uniformity and determine the shortest detection time. Next, the sensor is applied to detect two biomolecules, dopamine and urea, with unprecedented detection limit and speed compared to other existing microfluidic SERS sensors. The sensor holds great promise in point-of-care applications for various biomolecular detections.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
Citation Download Citation
Ming Li, Fusheng Zhao, Jianbo Zeng, Ji Qi, Jing Lu, and Wei-Chuan Shih "Microfluidic surface-enhanced Raman scattering sensor with monolithically integrated nanoporous gold disk arrays for rapid and label-free biomolecular detection," Journal of Biomedical Optics 19(11), 111611 (23 July 2014). https://doi.org/10.1117/1.JBO.19.11.111611
Published: 23 July 2014
JOURNAL ARTICLE
 8 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Lens.org Logo
CITATIONS
Cited by 82 scholarly publications.
Advertisement
Advertisement
KEYWORDS
Sensors
Microfluidics
Gold
Raman scattering
Urea
Surface enhanced Raman spectroscopy
Molecules
CHORUS Article. This article was made freely available starting 23 July 2015
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top