Ratio-based decisions and the quantitative analysis of cDNA microarray images

Yidong Chen; Edward R. Dougherty; Michael L. Bittner

doi:10.1117/12.281504

1 October 1997 Ratio-based decisions and the quantitative analysis of cDNA microarray images

Yidong Chen, Edward R. Dougherty, Michael L. Bittner

Author Affiliations +

Journal of Biomedical Optics, Vol. 2, Issue 4, (October 1997). https://doi.org/10.1117/12.281504

Abstract

Gene expression can be quantitatively analyzed by hybridizing fluor-tagged mRNA to targets on a cDNA microarray. Comparison of gene expression levels arising from cohybridized samples is achieved by taking ratios of average expression levels for individual genes. A novel method of image segmentation is provided to identify cDNA target sites and a hypothesis test and confidence interval is developed to quantify the significance of observed differences in expression ratios. In particular, the probability density of the ratio and the maximum-likelihood estimator for the distribution are derived, and an iterative procedure for signal calibration is developed.

Citation Download Citation

Yidong Chen, Edward R. Dougherty, and Michael L. Bittner "Ratio-based decisions and the quantitative analysis of cDNA microarray images," Journal of Biomedical Optics 2(4), (1 October 1997). https://doi.org/10.1117/12.281504

Published: 1 October 1997

Access the abstract

JOURNAL ARTICLE
11 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY