Optical reprogramming of human somatic cells using ultrashort Bessel-shaped near-infrared femtosecond laser pulses

Aisada Uchugonova; Hans Georg Breunig; Ana Batista; Karsten König

doi:10.1117/1.JBO.20.11.115008

30 November 2015 Optical reprogramming of human somatic cells using ultrashort Bessel-shaped near-infrared femtosecond laser pulses

Aisada Uchugonova, Hans Georg Breunig, Ana Batista, Karsten König

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Journal of Biomedical Optics, Vol. 20, Issue 11, 115008 (November 2015). https://doi.org/10.1117/1.JBO.20.11.115008

Abstract

We report a virus-free optical approach to human cell reprogramming into induced pluripotent stem cells with low-power nanoporation using ultrashort Bessel-shaped laser pulses. Picojoule near-infrared sub-20 fs laser pulses at a high 85 MHz repetition frequency are employed to generate transient nanopores in the membrane of dermal fibroblasts for the introduction of four transcription factors to induce the reprogramming process. In contrast to conventional approaches which utilize retro- or lentiviruses to deliver genes or transcription factors into the host genome, the laser method is virus-free; hence, the risk of virus-induced cancer generation limiting clinical application is avoided.

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Aisada Uchugonova, Hans Georg Breunig, Ana Batista, and Karsten König "Optical reprogramming of human somatic cells using ultrashort Bessel-shaped near-infrared femtosecond laser pulses," Journal of Biomedical Optics 20(11), 115008 (30 November 2015). https://doi.org/10.1117/1.JBO.20.11.115008

Published: 30 November 2015

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KEYWORDS

Femtosecond phenomena

Microscopes

Green fluorescent protein

Luminescence

Biomedical optics

Stem cells

Atomic force microscopy

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