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8 September 2016 Hairpin DNA-functionalized gold nanorods for mRNA detection in homogenous solution
Guoke Wei, Jun Yu, Jinliang Wang, Peng Gu, David J. Birch, Yu Chen
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Journal of Biomedical Optics, Vol. 21, Issue 9, 097001 (September 2016). https://doi.org/10.1117/1.JBO.21.9.097001
Abstract
We report a fluorescent probe for mRNA detection. It consists of a gold nanorod (GNR) functionalized with fluorophore-labeled hairpin oligonucleotides (hpDNA) that are complementary to the mRNA of a target gene. This nanoprobe was found to be sensitive to a complementary oligonucleotide, as indicated by significant changes in both fluorescence intensity and lifetime. The influence of the surface density of hpDNA on the performance of this nanoprobe was investigated, suggesting that high hybridization efficiency could be achieved at a relatively low surface loading density of hpDNA. However, steady-state fluorescence spectroscopy revealed better overall performance, in terms of sensitivity and detection range, for nanoprobes with higher hairpin coverage. Time-resolved fluorescence lifetime spectroscopy revealed significant lifetime changes of the fluorophore upon hybridization of hpDNA with targets, providing further insight on the hybridization kinetics of the probe as well as the quenching efficiency of GNRs.
© 2016 Society of Photo-Optical Instrumentation Engineers (SPIE) 1083-3668/2016/$25.00 © 2016 SPIE
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Guoke Wei, Jun Yu, Jinliang Wang, Peng Gu, David J. Birch, and Yu Chen "Hairpin DNA-functionalized gold nanorods for mRNA detection in homogenous solution," Journal of Biomedical Optics 21(9), 097001 (8 September 2016). https://doi.org/10.1117/1.JBO.21.9.097001
Published: 8 September 2016
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Cited by 19 scholarly publications.
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KEYWORDS
Luminescence
Nanoprobes
Nanorods
Gold
Fluorescence spectroscopy
Time resolved spectroscopy
Microelectromechanical systems
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